The association of HpDof1.7 and HpDof5.4 with soluble sugar accumulation in pitaya fruit by transcriptionally activating sugar metabolic genes  

作　　者：Zhenliang Mou Runxi Zeng Nanhui Chen Zongli Liu Zexiang Zeng Yonghua Qin Wei Shan Jianfei Kuang Wangjin Lu Jianye Chen Yating Zhao 牟真靓;曾闰曦;陈南慧;刘宗莉;曾泽湘;秦永华;单伟;邝健飞;陆旺金;陈建业;赵亚婷(State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation,Ministry of Education,College of Horticulture,South China Agricultural University,Guangzhou,China)

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation,Ministry of Education,College of Horticulture,South China Agricultural University,Guangzhou,China

出　　处：《Food Quality and Safety》2022年第4期619-628,共10页食品品质与安全研究（英文版）

基　　金：unded by the Key Science and Technology Planning Project of Guangzhou(No.201904020015);the Science and Technology Program of Guangzhou(No.202002020060);the Science and Technology Program of Zhanjiang(No.2019A01003),China.

摘　　要：Soluble sugar is one of the most important factors affecting fruit flavor and quality.Here,we report the identification of two Dof(DNA-binding with one finger)transcription factors termed HpDof1.7 and HpDof5.4 and their roles in influencing sugar accumulation in pitayas.HpDof1.7 and HpDof5.4 share a similar expression pattern with sugar metabolism-related genes HpSuSy1 and HpINV2,and sugar transporter genes HpTMT2 and HpSWEET14 during pitayas maturation,and their expression pattern was also consistent with the accumulation of glucose and fructose,which were the predominant sugars in pitayas.HpDof1.7 and HpDof5.4 were both typical nucleus-localized proteins with trans-activation ability.Gel mobility shift assay revealed that HpDof1.7 and HpDof5.4 were bound to promoters of HpSuSy1,HpINV2,HpTMT2 and HpSWEET14.Finally,transient expression assays in tobacco leaves showed that HpDof1.7 and HpDof5.4 increased the activities of HpSuSy1,HpINV2,HpTMT2 and HpSWEET14 promoters,thus facilitating sugar accumulation by transcriptionally enhancing sugar metabolic pathway genes.Our findings provide a new perspective on the regulatory mechanisms of Dof transcription factors in sugar accumulation and pitaya fruit quality formation.

关 键 词：PITAYA sugar accumulation Dof transcription factors transcriptional activation 

分 类 号：S66[农业科学—果树学]