Changes in fruit pigment accumulation,chloroplast development,and transcriptome analysis in the CRISPR/Cas9-mediated knockout of Stay-green 1(slsgr1)mutant  

作　　者：Liqun Ma Ni Zeng Ke Cheng Jinyan Li Keru Wang Chunjiao Zhang Hongliang Zhu 马力群;曾妮;成柯;李金焱;王珂茹;张春娇;朱鸿亮(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing,China;Supervision,Inspection&Testing Center of Agricultural Products Quality,Ministry of Agriculture and Rural Affairs,Beijing,China)

机构地区：[1]College of Food Science and Nutritional Engineering,China Agricultural University,Beijing,China [2]Supervision,Inspection&Testing Center of Agricultural Products Quality,Ministry of Agriculture and Rural Affairs,Beijing,China

出　　处：《Food Quality and Safety》2022年第1期3-12,共10页食品品质与安全研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.31972472 and No.32061143022).

摘　　要：The green-flesh(gf)mutant of the tomato fruit ripen to a muddy brown color and has been demonstrated previously to be a loss-of-function mutant.Here,we provide more evidence to support this view that S/SGR1 is involved in color change in ripening tomato fruits.Knocking out SISGR1 expression using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 genome editing strategy showed obviously a muddy brown color with significantly higher chlorophyll and carotenoid content compared with wild-type(WT)fruits.To further verify the role of SiSGR1 in fruit color change,we performed transcriptome deep sequencing(RNA-seq)analysis,where a total of 354 differentially expressed genes(124/230 downregulated/upregulated)were identified between WT and slsgr1.Additionally,the expression of numerous genes associated with photosynthesis and chloroplast function changed significantly when S/SGR1 was knocked out.Taken together,these results indicate that S/SGR1 is involved in color change in ripening fruit via chlorophyll degradation and carotenoid biosynthesis.

关 键 词：TOMATO pigmentaccumulation transcriptome analysis 

分 类 号：Q94[生物学—植物学]