镉对嗜热小球藻的毒性效应及生物富集研究  

Toxic Effects of Cadmium on Chlorella thermophila and Its Bioaccumulation

作　　者：裴福文 谢作明 毛青[1] 李鎏爽 钟兆淇 赵欣鑫 PEI Fuwen;XIE Zuoming;MAO Qing;LI Liushuang;ZHONG Zhaoqi;ZHAO Xinxin(School of Environmental Studies,China University of Geosciences(Wuhan),Wuhan 430078,China;State Key Laboratory of Biogeology and EnvironmentaI Geology,China University of Geosciences(Wuhan),Wuhan 430078,China)

机构地区：[1]中国地质大学(武汉)环境学院,湖北武汉430078 [2]中国地质大学(武汉)生物地质与环境地质国家重点实验室,湖北武汉430078

出　　处：《安全与环境工程》2023年第2期163-172,共10页Safety and Environmental Engineering

基　　金：国家自然科学基金面上项目(41572230、41172219);国家自然科学基金创新研究群体项目(41521001)。

摘　　要：藻类在重金属生物修复中具有巨大的潜力,探究藻-镉之间的相互作用,确定藻株性能,具有重要的意义。从矿区污染环境中筛选得到一株嗜热小球藻(Chlorella thermophila),考察不同Cd^(2+)浓度胁迫下嗜热小球藻的生理生化特性及其镉富集能力。结果表明:嗜热小球藻对Cd^(2+)具有较强的耐受能力,嗜热小球藻的96 h半效应浓度(EC50-96 h)为8.969 mg/L;随着Cd^(2+)浓度的增加,小球藻细胞内的光合色素含量逐渐降低;在小球藻藻细胞内抗氧化系统中超氧化物歧化酶(SOD)含量呈先增加后降低的趋势,7 mg/L Cd^(2+)浓度下小球藻藻细胞内抗氧化系统中SOD的含量最高,说明过高的Cd^(2+)浓度会导致小球藻藻细胞抗氧化系统受损,而丙二醛(MDA)含量逐渐增加,说明小球藻藻细胞损伤明显;小球藻对Cd^(2+)具有较好的吸附性能,3 mg/L Cd^(2+)浓度下第6天时小球藻对Cd^(2+)的吸附效率可达63.78%。可见,藻与镉之间存在相互作用,高浓度Cd^(2+)会对嗜热小球藻的生长、光合系统及抗氧化系统造成一定的损害,但总体来看,嗜热小球藻耐镉性能较好,同时具有良好的镉生物富集能力。Algae strains have great potential in the bioremediation of heavy metal.It’s of great significance to explore the interaction between algae and cadmium and determine the performance of algae strains・A strain of Chlorella thermophila is screened from the polluted environment in the mining area 9 and the physiological-biochemical characteristics and cadmium(Cd)accumulation ability of the algae under different Cd Cd^(2+)concentrations are investigated.The results show that Chlorella thermophila has good tolerance to Cd^(2+),and the median effect concentration(EC_(50)-96 h)is 8.969 mg/L・With the increase of Cd^(2+)concentration,the contents of photosynthetic pigments in algal cells decrease gradually.In the antioxidant system of algal cells,the superoxide dismutase(SOD)content increases initially and then decreases,and the content of SOD is the highest at 7 mg/L Cd^(2+)concentration,which means excessive Cd^(2+)concentration causes damage to the antioxidant systemof algal cells;but the content of malondialdehyde(MDA)increases gradually,indicating that the algal cells show obvious damage・Chlorella thermophila has high adsorption capacity for cadmium,and the adsorption capacity can reach 63.78%at the concentration of 3 mg/L Cd^(2+)on the 6 th day.In conclusion,there's an interaction between algae and cadmium,and high concentration of Cd^(2+)can cause certain damage to the growth,photosynthetic and antioxidant systems of algae.However,in general,Chlorella thermophila has high cadmium tolerance and good cadmium bioaccumulation ability.

关 键 词：嗜热小球藻 镉 毒性效应 生物富集 

分 类 号：X172[环境科学与工程—环境科学]