过表达Pseudomonas mendocina复制起始相关蛋白提高菌体繁殖速度  

Overexpression of Pseudomonas mendocina Replication-associated Protein Increases Bacterial Growth Rate

作　　者：范旭 赵风杰 乔明强[1] 杨超[1] Fan Xu;Zhao Fengjie;Qiao Mingqiang;Yang Chao(Key Laboratory of Molecular Microbiology and Technology for Ministry of Education,Nankai University,Tianjin 300071,China)

机构地区：[1]南开大学分子微生物与技术教育部重点实验室,天津300071

出　　处：《南开大学学报（自然科学版）》2023年第1期19-25,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金面上项目(31470123)。

摘　　要：复制起始蛋白DnaA通过识别复制起始位点oriC上的特殊序列,并招募复制复合体起始复制,它通过绑定ATP或ADP来对复制起始的开始进行调节.DNA聚合酶Ⅲ的β亚基DnaN蛋白,负责将DNA聚合酶III连接到已经解旋的DNA上,以开始复制过程.通过过表达复制起始相关蛋白DnaA和DnaN,促进了菌体的繁殖能力,优化了菌体的生长状态,缩短了菌体倍增时间,为构建Pseudomonas mendocina工程菌株提供了参考.The replication initiation protein DnaA initiates replication by recognizing a specific sequence on the replication initiation site oriC and recruiting a replication complex that regulates the onset of replication initiation by binding ATP or ADP.The beta subunit DnaN protein of DNA polymerase III is responsible for linking DNA polymerase Ⅲ to the DNA that has been untwisted to initiate the replication process.In this experiment,the replication-initiating related proteins DnaA and DnaN were over-expressed,which promoted the reproductive capacity of the cells,optimized the growth state of the cells,shortened the cell generation time,and provided a reference for constructing the Pseudomonas mendocina engineering strain.

关 键 词：Pseudomonas mendocina 复制起始蛋白 生长速度 扫描电镜 菌体倍增时间 

分 类 号：Q93[生物学—微生物学]