Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy  

作　　者：Xinhai Zhou Dawei Zhou Xinhui Bao Yang Zhang Jie Zhou Fengxue Xin Wenming Zhang Xiujuan Qian Weiliang Dong Min Jiang Katrin Ochsenreither 

机构地区：[1]State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China [2]Institute of Microbiology,Chinese Academy of Science,Beijing 100101,China [3]Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM),Nanjing Tech University,Nanjing 211800,China [4]Institute of Process Engineering in Life Sciences,Section Ⅱ:Technical Biology,Karlsruhe Institute of Technology,Fritz-Haber-Weg4,Karlsruhe 76131,Germany

出　　处：《Chinese Journal of Chemical Engineering》2023年第1期324-331,共8页中国化学工程学报（英文版）

基　　金：supported by the National Key Research & Development Program of China (2021YFC2101500, 2018YFA0902200);National Natural Science Foundation of China (22008115, 21978130);Jiangsu Province Natural Science Foundation for Youths (SBK2020044721);Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund Project (CX(21)3120);Jiangsu Planned Projects for Postdoctoral Research Funds (2021K085A);China Postdoctoral Science Foundation (2020M671467);Postdoctoral Research Funding Program of Jiangsu Province (2021K085A)

摘　　要：Palmitoleic acid(POA)can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry.Recently,the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified with high content of POA in its intracellular lipid(13.80%).In this study,process optimization focused on dissolved oxygen regulation to improve POA production was conducted.Dynamic agitation was found to do significant enhancement on POA-rich lipid production than aeration regulation.Under the best condition of 1000 r·min^(-1)of agitation and 1 vvm(airvolume/culture volume/min)of aeration,no ethanol was detected during the whole fermentation process,while a dry biomass concentration of 44.80 g·L^(-1)with 13.43 g·L^(-1)of lipid and 2.93 g·L^(-1)of POA was achieved.Transcription analysis revealed that the ethanol synthetic pathway was downregulated under the condition of high agitation,while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.

关 键 词：Palmitoleic acid production Scheffersomyces segobiensis Dissolved oxygen ETHANOL BIOPROCESS Bioreactors 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]