肉桂醛对PC-3细胞增殖、EMT及干细胞特性的影响  

Effects of cinnamaldehyde on proliferation,EMT and stem cell characteristics of PC-3 cells

作　　者：吴俊檄 唐欲博[2] 令狐熙涛 包广龙 施浩然 文振宇 黄帅 瓦庆德 WU Junxi;TANG Yubo;LINGHU Xitao;BAO Guanglong;SHI Haoran;WEN Zhenyu;HUANG Shuai;WA Qingde(Department of Orthopedics,the Second Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China;不详)

机构地区：[1]遵义医科大学第二附属医院骨外科,贵州遵义563000 [2]中山大学附属第一医院药学部,广州510260 [3]广州医科大学附属第二医院骨外科,广州510260

出　　处：《实用医学杂志》2023年第3期298-302,308,共6页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:82160577);广州市科技计划项目(编号:202102010071);贵州省科技计划项目(编号:黔科合基础-ZK[2021]重点007);贵州省优秀青年科技人才培养项目(编号:黔科合平台人才[2021]5613号)。

摘　　要：目的 探索肉桂醛(cinnamaldehyde,CA)对PC-3细胞增殖、上皮-间质转化(epithelial-mesenchymal transition,EMT)及干细胞特性的作用及机制,为CA作为潜在抗前列腺癌骨转移药物提供前期实验参考。方法 通过CCK-8法、Transwell实验、基质黏附实验及成球实验检测CA对PC-3细胞增殖、迁移、侵袭、黏附及成球的影响,qRT-PCR分析CA处理PC-3细胞后miR-143及AGO2 mRNA表达情况,Transwell实验和基质黏附实验检测单独加入CA、转染miR-143mimic、AGO2-siRNA同时处理CA及AGO2、同时转染miR-143mimic及AGO2后PC-3细胞的侵袭、迁移及黏附情况。Western blot实验检测CA或转染miR-143mimic后PC-3细胞AGO2、EMT及干细胞特性相关蛋白的表达情况。结果 CA呈浓度依赖性抑制PC-3细胞的增殖、迁移、侵袭、黏附及成球能力,与对照组相比差异均有统计学意义(P <0.01);qRT-PCR分析发现CA可上调PC-3细胞中miR-143表达、抑制AGO2 mRNA表达;过表达miR-143及沉默AGO2均能抑制PC-3细胞的迁移、侵袭及黏附,而同时上调AGO2及CA、同时上调miR-143及AGO2后PC-3细胞迁移、侵袭及黏附的抑制作用并不明显。此外,过表达miR-143及CA均能抑制PC-3细胞中AGO2、ZEB1、Vimentin、N-cadherin、fibronectin及CD44、Oct-4、c-Myc蛋白的表达。结论 CA可在体外抑制PC-3细胞的增殖、迁移、侵袭及黏附能力,此外,CA可抑制PC-3细胞的EMT及干细胞特性,其机制可能与其能上调miR-143/AGO2轴有关。Objective To explore the effects of cinnamaldehyde(CA)on the proliferation,epithelialmesenchymal transition(EMT)and stem cell characteristics of PC-3 cells and their mechanisms,so as to provide reference for preliminary experiment of CA as a potential drug preventing bone metastasis in prostate cancer.Methods The effects of CA on the proliferation,migration,invasion,adhesion and spheres formation of PC-3 cells were detected using CCK8,Transwell,matrix adhesion and spheres formation assays.The expressions of miR-143 and AGO2 mRNA in PC-3 cells after CA treatment were determined by qRT-PCR,while the effects on the invasion,migration and adhesion of PC-3 cells in the single CA,single transfection of miR-143mimic or AGO2-siRNA,simultaneous treatment of CA and AGO2,or miR-143 mimic and AGO2 group were detected by Transwell and matrix adhesion assays.Furthermore,the protein expressions of CA or transfection of miR-143 mimic on EMT and stem cell characteristics of PC-3 cells were determined using western blot.Results CA inhibited the proliferation,migration,invasion,adhesion and spheroid of PC-3 cells in a concentration-dependent manner,showing statistically significant differences while compared with that in the control group(P 0.01).qRT-PCR showed that CA up-regulated the expression of miR-143 and down-regulate the expression of AGO2 mRNA in PC-3 cells.Overexpression of miR-143 or down-regulation of AGO2 inhibited the migration,invasion and adhesion of PC-3 cells.Also,the inhibition on the invasion,migration and adhesion of PC-3 cells was not obvious after the simultaneous up-regulation of AGO2 and CA or miR-143 and AGO2.In addition,CA and overexpression of miR-143 inhibited the expressions of AGO2,ZEB1,vimentin,N-cadherin,fibronectin,CD44,Oct-4 and c-Myc proteins in PC-3 cells.Conclusion CA inhibits the proliferation,migration,invasion and adhesion of PC-3 cells in vitro;it also inhibits the EMT and stem cell characteristics of PC-3 cells,and its mechanism may be related to its ability to up-regulate the miR-143/AGO2

关 键 词：肉桂醛 PC-3 MIR-143 上皮-间质转化 干细胞特性 

分 类 号：R738.1[医药卫生—肿瘤]