Russian Collaborative Development of Reproduction Technologies for the Sustainable Management of Amphibian Biodiversity  

作　　者：Victor K.UTESHEV Edith N.GAKHOVA Ludmila I.KRAMAROVA Natalia V.SHISHOVA Svetlana A.KAUROVA Elena A.KIDOVA Artem A.KIDOV Robert K.BROWNE 

机构地区：[1]Institute of Cell Biophysics,Russian Academy of Sciences,Pushchino,Moscow 142290,Russia [2]Institute of Theoretical and Experimental Biophysics,Russian Academy of Sciences,Pushchino,Moscow 142290,Russia [3]Russian State Agrarian University-K.A.Timiryazev Agricultural Academy,Moscow 127550,Russia [4]Sustainability America,La Isla Road,Corozal District,Belize

出　　处：《Asian Herpetological Research》2023年第1期103-115,共13页亚洲两栖爬行动物研究（英文版）

基　　金：performed within the framework of State projects 122041100276-0 and 075-01027-2200。

摘　　要：Reproduction technologies(RTs)can provide for the reliable reproduction of amphibians,as well as perpetuation of species genetic variation with the use of biobanks.In 1982,in anticipation of the biodiversity conservation crisis,major Russian institutions collaborated in a dynamic program to develop and implement RTs for the sustainable management of amphibian biodiversity.An initial primary focus was the captive breeding of threatened Russian endemic anuran and caudate species,using RTs that varied from environmental manipulation to the use of exogenous gonadotropic hormones to stimulate reproduction.These species were mostly from Palearctic or cool mountain regions,but also included a wide range of species from warm regions.Other early achievements included the successful cryopreservation of anuran spermatozoa and anuran diploid pluripotent cell nuclei,in order to store both the matrilineal and patrilineal genomes in biobanks,with their subsequent development to the blastula stage after implantation into enucleated oocytes.After the turn of the 21st Century,in support of the priorities of the Amphibian Conservation Action Plan(2007),we developed RTs for the refrigerated storage of testicular or urinary spermatozoa for days to weeks at 4℃,the cryopreservation of urinary spermatozoa using anovel cryoprotectant,the in vitro fertilisation of hormonally induced oocytes either fresh or after refrigerated ex situ or in situ storage,and the artificial insemination of salamanders with fresh spermatozoa.In this article,we describe previously unpublished techniques and techniques from obscure Russian sources.

关 键 词：artificial fertilisation assisted reproductive technologies(ART) CRYOPRESERVATION gonadotropic hormones OOCYTES SPERMATOZOA storage urinary spermatozoa 

分 类 号：Q958[生物学—动物学]