食管鳞状细胞癌组织中RSK4、p63的表达变化及意义  

Expression and significance of RSK4 and p63 in esophageal squamous-cell carcinoma

作　　者：刘艳红 张哲[2] 王哲 王景杰 LIU Yanhong;ZHANG Zhe;WANG Zhe;WANG Jingjie(Department of Rehabilitation and Physiotherapy of Traditional Chinese Medicine,The 74th Group Military Hospital of People's Liberation Army Ground Force,Guangzhou 510318,China;不详)

机构地区：[1]解放军陆军第七十四集团军医院中医康复理疗科,广州510318 [2]解放军总医院海南医院消化内科 [3]空军军医大学西京医院病理科 [4]空军军医大学唐都医院消化内科

出　　处：《山东医药》2023年第10期16-20,共5页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81272651)。

摘　　要：目的探讨食管鳞状细胞癌(鳞癌)组织中核糖体S6蛋白激酶4(RSK4)、p63的表达变化及意义。方法收集食管鳞癌活检标本72例份,应用免疫组织化学EnVision两步法检测癌组织中RSK4、p63蛋白表达,染色结果采用半定量分析。选取食管鳞状细胞癌细胞系TE-11、TE-10及正常食管细胞Het-1A,分别采用qRT-PCR法、Western blotting法检测RSK4、p63 mRNA及蛋白表达。采用Kaplan-Meier法及Cox风险回归模型对患者进行生存分析。结果食管鳞状细胞癌组织中RSK4蛋白阳性表达率为62.5%(45/72),p63蛋白阳性表达率为83.3%(60/72)。食管鳞状细胞癌细胞系(TE-11、TE-10)中RSK4、p63基因的mRNA和蛋白表达均高于正常食管细胞Het-1A(P均<0.05)。Kaplan-Meier法生存分析结果显示,RSK4阳性表达与食管癌患者预后不良相关(P<0.05),且分期、组织分化程度、淋巴结转移等均是影响患者生存期的因素(P均<0.05);p63表达与患者临床病理特征及预后均无关(P均>0.05)。Cox回归模型分析结果显示,RSK4阳性表达、pT分期、组织分化程度、淋巴结转移与食管癌患者生存有关(P均<0.05)。结论RSK4与p63在食管鳞状细胞癌组织中呈异常表达,RSK4可作为潜在食管癌分期的分子标志物及预后评估因子。Objective To investigate the expression and significance of ribosomal S6 protein kinase 4(RSK4)and p63 in esophageal squamous-cell carcinoma.Methods The expression of RSK4 and p63 in 72 cases of esophageal squamous-cell carcinoma was detected by EnVision immunohistochemical method.Semiquantitative analysis was conducted on staining results.The mRNA and protein expression levels of RSK4 and p63 in the human esophageal squamous-cell carcinoma cell lines TE-11,TE-10,and normal esophageal cells Het-1A were detected by qRT-PCR and Western blotting,respectively.Kaplan-Meier method and Cox regression model were used to analyze the survival of the patients.Results The positive protein expression rates of RSK4 and p63 in esophageal squamous-cell carcinoma were 62.5%(45/72)and 83.3%(60/72),respectively.The mRNA and protein expression levels of RSK4 and p63 genes in esophageal squamouscell carcinoma cell lines TE-11 and TE-10 were higher than those in normal esophageal cells Het-1A(all P<0.05).Kaplan-Meier survival analysis showed that the positive expression of RSK4 was associated with poor prognosis(P<0.05),and staging,histological differentiation,and lymph node metastasis were factors affecting the survival(all P<0.05).The expression of p63 was not correlated with clinicopathological features or prognosis(both P>0.05).Cox regression analysis showed that the positive expression of RSK4,pT stage,histological differentiation,and lymph node metastasis were related to the survival of patients with esophageal carcinoma(all P<0.05).Conclusions RSK4 and P63 are abnormally ex‑pressed in esophageal squamous-cell carcinoma.RSK4 can be used as a molecular marker for staging and prognostic as-sessment of potential esophageal carcinoma.

关 键 词：食管鳞状细胞癌 核糖体S6蛋白激酶4 P63基因 生存分析 

分 类 号：R735.1[医药卫生—肿瘤]