基于TCGA数据库分析甲状腺癌微环境中免疫细胞组分及免疫调节因子变化  

Analysis on changes of immune cell components and immunoregulatory factors in thyroid cancer microenvironment based on TCGA database

作　　者：何魏 程岳 张勐 占天鹏 周剑辉 陶燕[3] 李兰兰[3] 卢建中[3] 付生军[3] 张静[3] 刘善辉 HE Wei;CHENG Yue;ZHANG Meng;ZHAN Tianpeng;ZHOU Jianhui;TAO Yan;LI Lanan;LU Jianzhong;FU Shengjun;ZHANG Jing;LIU Shanhui(Lanzhou University Second Hospital,Gansu,Lanzhou 730000,China;Department of Respiratory Medicine andEndocrinology,Shihezi City People’s Hospital,Xinjiang,Shihezi 832061,China;Institute of Urology,Lanzhou UniversitySecond Hospital,Gansu,Lanzhou 730000,China)

机构地区：[1]兰州大学第二临床医学院,甘肃兰州730000 [2]新疆石河子市人民医院呼吸内分泌科,新疆石河子832061 [3]兰州大学第二医院泌尿外科研究所,甘肃兰州730000

出　　处：《中国医药科学》2023年第5期37-40,52,共5页China Medicine And Pharmacy

摘　　要：目的 探索分析甲状腺癌微环境中免疫细胞浸润,并评估其同甲状腺癌临床预后等的关系。方法 在TCGA数据库下载甲状腺癌患者临床数据,计算样本中22种免疫细胞含量,采用t检验评估癌旁组织和肿瘤组织中22种免疫细胞组分和多种免疫调节因子表达差异。结果 肿瘤组织中静息态树突状细胞、M0型巨噬细胞、M2型巨噬细胞和静息态肥大细胞含量较高;而M1型巨噬细胞、B细胞、浆细胞、CD4^(+)T细胞、CD8^(+)T细胞、Tfh细胞和γδT细胞含量较低。免疫检查点分子和趋化因子表达水平在甲状腺癌组织和癌旁组织中显著差异表达。结论 本研究确定了甲状腺癌中的多种免疫细胞和免疫调节因子的表达差异,有可能作为甲状腺癌治疗和预后的潜在靶点。Objective To explore and analyze the immune cell infiltration in thyroid cancer microenvironment and evaluate its relationship with the clinical prognosis of thyroid cancer.Methods The clinical data of patients with thyroid cancer were downloaded from TCGA database,the content of 22 kinds of immune cells in the samples was calculated,and the expression differences of 22 kinds of immune cell components and multiple immunoregulatory factors in the tumor tissues and adjacent tissues were evaluated by t-test.Results The resting dendritic cells,M0 macrophages,M2 macrophages and resting mast cells were highly expressed in tumor tissues.M1 macrophages,B cells,plasma cells,CD4^(+)T cells,CD8^(+)T cells,Tfh cells andγδT cells were expressed less in tumor tissues.The expression levels of immune checkpoint molecules and chemokines were significantly different between thyroid cancer tissues and adjacent tissues.Conclusion This study has determined the expression differences of various immune cells and immunoregulatory factors in thyroid cancer,which may be a potential target for the treatment and prognosis of thyroid cancer.

关 键 词：甲状腺癌 免疫细胞 免疫调节因子 预后 

分 类 号：R735.2[医药卫生—肿瘤]