‘海尔特兹’红树莓果实发育过程中NLPs(NIN-like proteins)转录因子家族的生物信息学分析  

Bioinformatics analysis of NLPs(NIN-like proteins)transcription factor family during fruit development of ‘Heritage’red raspberry

作　　者：范青 郑奕宸 梁光毅 李明[1] 张雪梅[2] 赵志磊[3] 李寒[2] 顾玉红[1] FAN Qing;ZHENG Yichen;LIANG Guangyi;LI Ming;ZHANG Xuemei;ZHAO Zhilei;LI Han;GU Yuhong(College of Life Science,Hebei Agricultural University,Baoding 071001,China;College of Forestry,Hebei Agricultural University,Baoding 071001,China;College of Quality and Technical Supervision,Hebei University,Baoding 071002,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学林学院,河北保定071001 [3]河北大学质量技术监督学院,河北保定071002

出　　处：《河北农业大学学报》2023年第2期1-7,共7页Journal of Hebei Agricultural University

基　　金：河北省重点研发计划项目(20326338D)。

摘　　要：为探究‘海尔特兹’红树莓中NLPs(NIN-like proteins)转录因子家族成员的基本特征和表达模式,本研究利用生物信息学技术对‘海尔特兹’红树莓4个发育时期(青果、黄果、红果、深红果)果实的NLP基因家族成员进行分析。结果表明:红树莓中3个NLPs转录因子都含有RWP-RK结构域,其中RuNLP1和RuNLP2的保守基序相同;红树莓的NLPs转录因子家族成员与野草莓和月季的NLPs转录因子家族进化关系最近,且NLPs基因家族的基因扩增发生在单子叶植物和双子叶植物祖先分化之前;RuNLP1和RuNLP2为酸性不稳定的亲水性蛋白质,而RuNLP3为碱性不稳定的亲水性蛋白质;三者都不含信号肽和跨膜结构;RuNLP1和RuNLP2蛋白质均定位于细胞核,而RuNLP3定位于细胞核和叶绿体;二级结构的预测结果显示,结构占比均以无规卷曲为主,α螺旋次之,与三级结构预测结果基本一致,结果较为可靠;RuNLPs转录因子家族成员之间不存在互作现象;RuNLPs基因家族3个成员的表达量随果实逐渐成熟均减少。本研究为RuNLPs转录因子家族在红树莓果实发育过程中的分子机制提供了理论基础。In order to explore the basic characteristics and expression patterns of NLPs(NIN-like proteins)in‘Heritage’red raspberry,bioinfonnatic analysis was applied on the NLP gene family members in the fruits of red raspberry Heritage at four developmental stages(green fruit,yellow fruit,red fruit and dark red fruit).The results showed that three NLP transcription factors in red raspberry contained RWP-RK domain,among which RuNLPl and RuNLP2 harbored the same conserved motif The NLPs transcription factor family members of red raspberry have the closest evolutionary relationship with the NLPs transcription factor family members of wild strawberry and Chinese rose,and the gene amplification of NLP gene family occurred before the divergence of monocot and dicot ancestors.RuNLPl and RuNLP2 were acidic unstable hydrophilic proteins,while RuNLP3 was alkaline unstable hydrophilic proteins.None of them contained signal peptides or transmembrane structures.RuNLPl and RuNLP2 proteins were localized in the nucleus,while RuNLP3 was localized in the nucleus and chloroplast.The prediction results showed that the secondary structure was mainly random coil followed by alpha-helix,which was basically consistent with the prediction results of tertiary structure suggesting that the results were relatively reliable.RuNLP transcription factor family members did not interact with each other.The expression levels of three RuNLP genes decreased with fruit ripening.This study provided a theoretical basis for the molecular mechanism of RuNLPs family in red raspberry fruit development.

关 键 词：红树莓 果实 发育 NLPs转录因子家族 生物信息学分析 

分 类 号：Q781[生物学—分子生物学]