上皮性卵巢癌组织CDCA7表达水平及其与患者预后的相关性研究  

Expression Level of CDCA7 in Epithelial Ovarian Cancer Tissues and Its Correlation with Prognosis of Patients

作　　者：李苗[1] 龚姗[1] 金海红[1] 付静静[1] 韩坤 LI Miao;GONG Shan;JIN Hai-hong;FU Jing-jing;HAN Kun(Department of Gynaecology,the First Hospital of Qinhuangdao City,Hebei Qinhuangdao 066000,China)

机构地区：[1]秦皇岛市第一医院妇科,河北秦皇岛066000

出　　处：《现代检验医学杂志》2023年第2期118-123,共6页Journal of Modern Laboratory Medicine

基　　金：河北省医学科学研究课题计划项目(20221606);秦皇岛市科学技术研究与发展项目(202101A066)。

摘　　要：目的探讨上皮性卵巢癌(epithelial ovarian cancer,EOC)组织细胞分裂周期相关蛋白(cell division cycle associated protein 7,CDCA7)表达水平及其与患者预后的相关性。方法收集2010年10月~2020年10月河北省秦皇岛市第一医院收治的90例EOC患者癌组织标本和50例癌旁组织标本,采用免疫组织化学法检测癌组织、癌旁组织标本CDCA7阳性表达情况;采用实时荧光定量(qRT-PCR)法检测癌组织、癌旁组织标本CDCA7 mRNA相对表达量。比较EOC癌组织标本中CDCA7表达情况与临床病理特征的关系;Kaplan-Meier法分析EOC癌组织标本中CDCA7表达情况与患者无进展生存期(progression-free survival,PFS)和生存率的相关性以及COX风险比例回归分析影响预后的因素。结果CDCA7蛋白在EOC癌组织中阳性表达率为78.33%,高于癌旁组织的64.17%,差异有统计学意义(χ^(2)=70.767,P=0.000);CDCA7mRNA在癌组织中的相对表达量为6.05±0.32,高于癌旁组织(1.08±0.06),差异有统计学意义(t=108.600,P=0.000);CDCA7高表达与EOC患者病理分期、肿瘤浸润深度、病理分型、淋巴结转移、CA125水平和HE4水平等临床特征有关(χ^(2)=3.947~8.420,均P<0.05);Kaplan-Meier法生存分析,CDCA7低表达EOC患者PFS生存时间为98.15±10.72个月,高于CDCA7高表达患者(63.25±7.49个月),差异有统计学意义(Log-Rankχ^(2)=3.849,P=0.049);CDCA7低表达患者12个月的总生存率为87.35%,高于CDCA7高表达患者的45.76%,差异有统计学意义(Log-Rankχ^(2)=6.905,P=0.012);COX比例风险回归,TNM分期为Ⅲ~Ⅳ期、并发淋巴结转移、CDCA7高表达为影响患者12个月PFS的独立危险因素(Waldχ^(2)=4.858,4.892,5.025,P=0.028,0.028,0.026)。结论CDCA7在EOC患者癌组织中阳性表达率高于癌旁组织,CDCA7高表达是影响患者12个月PFS的独立危险因素,可作为EOC患者不良预后评估的分子标志物。Objective To explore the expression level of cell division cycle associated protein 7(CDCA7)in epithelial ovarian cancer(EOC)tissues and its correlation with prognosis of patients.Methods The cancer tissue samples from 90 EOC patients and para-cancerous tissue samples from 50 EOC patients admitted to the First Hospital of Qinhuangdao City were collected between October 2010 and October 2020.The positive expression of CDCA7 in cancer tissues and para-cancerous tissues was detected by immunohistochemistry.The relative expression level of CDCA7 mRNA in cancer tissues and para-cancerous tissues was detected by real-time fluorescence quantitative PCR(qRT-PCR).The relationship between CDCA7 in EOC tissues and clinicopathological characteristics was compared.The correlation between CDCA7 in EOC tissues and progression-free survival(PFS),survival rate was analyzed by Kaplan-Meier.The influencing factors of prognosis were analyzed by COX hazard proportional regression model.Results The positive expression rate of CDCA7 protein in EOC tissues was higher than that in para-cancerous tissues(78.33%vs 64.17%),the difference was statistically significant(χ^(2)=70.767,P=0.000).The relative expression level of CDCA7 mRNA in cancer tissues was higher than that in para-cancerous tissues(6.05±0.32 vs 1.08±0.06),the difference was statistically significant(t=108.600,P=0.000).The high-expression CDCA7 was correlated with pathological staging,tumor invasion depth,pathological classifications,lymph node metastasis,CA125 and HE4 levels(χ^(2)=3.947~8.420,all P<0.05).Kaplan-Meier survival analysis showed that PFS in EOC patients with low-expression CDCA7 was longer than that with high-expression CDCA7(98.15±10.72 months vs 63.25±7.49 months),the difference was statistically significant(Log-Rankχ^(2)=3.849,P=0.049)and 12-month overall survival rate was higher than that with high-expression CDCA7(87.35%vs 45.76%)and the difference was statistically significant(Log-Rankχ^(2)=6.905,P=0.012).COX hazard proportional regression model show

关 键 词：上皮性卵巢癌 细胞分裂周期相关蛋白7 

分 类 号：R737.31[医药卫生—肿瘤] R730.43[医药卫生—临床医学]