冷藏温度对日本刀角瓢虫存活及生长发育的影响  

Effects of cold-storage temperature on the survival and growth and development of Serangium japanicum(Coleoptera:Coccinellidae)

作　　者：曹惠怡 罗小龙 韦雪媛 梁建锋 谢永辉[2] 王兴民[1] CAO Hui-Yi;LUO Xiao-Long;WEI Xue-Yuan;LIANG Jian-Feng;XIE Yong-Hui;WANG Xing-Min(Engineering Research Center of Biological Control of Agricultural Pests of Guangdong Province,Key Laboratory of Bio-Pesticide innovation and Application of Guangdong Province,Department of Entomology,College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;Yunnan Tobacco Company Kunming Branch,Kunming 650201,China)

机构地区：[1]华南农业大学植物保护学院昆虫学系,广东省生物农药创制与应用重点实验室,广东省农业害虫生物防治工程研究中心,广州510642 [2]云南省烟草公司昆明市公司,昆明650201

出　　处：《昆虫学报》2023年第2期226-234,共9页Acta Entomologica Sinica

基　　金：中国烟草总公司重大科技项目(110202101053(LS-13));云南省烟草公司科技计划项目(2021530000242001,2021530000242035);广州市科技计划项目(202206010113)。

摘　　要：【目的】本研究旨在调查不同冷藏温度下日本刀角瓢虫Serangium japanicum成虫生物学特性及F 1代的生长发育,明确日本刀角瓢虫成虫最适冷藏温度。【方法】将日本刀角瓢虫成虫置于不同低温(7,10,13和16℃),贮藏10 d时测定其存活率、单雌产卵量、寿命和对烟粉虱Bemisia tabaci 4龄若虫的日捕食量,以及日本刀角瓢虫F 1代存活率和发育历期;qRT-PCR测定日本刀角瓢虫成虫体内热激蛋白基因Hsp 70和Hsp 90的相对表达量。【结果】日本刀角瓢虫成虫在16℃低温贮藏10 d时,其存活率、雌雄成虫寿命、单雌产卵量和日捕食量以及F 1代存活率均与贮藏在26℃的对照无显著差异(存活率:99%vs 100%;雌成虫寿命:110.65 d vs 106.87 d;雄成虫寿命:123.12 d vs 108.79 d;单雌产卵量:399.19粒vs 422.63粒;日捕食量:34.70头vs 31.95头;F 1代存活率:80.39%vs 94.12%);但其F 1代发育历期与对照相比显著缩短(17.33 d vs 18.89 d)。7℃贮藏10 d时,日本刀角瓢虫成虫全部死亡;10℃贮藏时,其存活率、单雌产卵量和F 1代存活率受到显著影响;13℃贮藏10 d时单雌产卵量受到显著影响。各贮藏低温均能诱导日本刀角瓢虫Hsp 70和Hsp 90的表达量上调,且温度越低,相对表达量越高;10℃处理组成虫体内Hsp 70和Hsp 90的相对表达量分别为对照的7.06和2.33倍。【结论】综合以上研究结果,日本刀角瓢虫最适冷藏温度为16℃,热激蛋白Hsp70和Hsp90可能在日本刀角瓢虫成虫响应低温胁迫时发挥了保护作用。【Aim】This study aims to investigate the biological characteristics of adults and the growth and development of F 1 generation of Serangium japonicum at different cold-storage temperatures,so as to determine the optimal cold-storage temperature of S.japonicum adults.【Methods】S.japonicum adults were stored at different low temperatures(7,10,13 and 16℃),their survival rates,numbers of eggs laid per female,longevity and daily predation amount against the 4th instar nymphs of Bemisia tabaci,the survival rates and developmental duration of F 1 generation of S.japonicum,and the relative expression levels of the heat shock protein genes Hsp 70 and Hsp 90 in S.japonicum adults were determined at 10 d after storage by qRT-PCR.【Results】At 10 d after S.japonicum adults were stored at 16℃,their survival rate,female and male adult longevity,number of eggs laid per female and daily predation amount,and the survival rate of F 1 generation were not significantly different from those of the control stored at 26℃(survival rate:99%vs 100%;female adult longevity:110.65 d vs 106.87 d;male adult longevity:123.12 d vs 108.79 d;number of eggs laid per female:399.19 grains vs 422.63 grains;daily predation amount:34.70 individuals vs 31.95 individuals;F 1 progeny survival rate:80.39%vs 94.12%);but the developmental duration of F 1 generation was significantly shorter than that of the control(17.33 d vs 18.89 d).At 10 d after cold-storage,all S.japonicum adults died when stored at 7℃,when stored at 10℃,their survival rate and number of eggs laid per female and the survival rate of F 1 generation were significantly affected,and when stored at 13℃,the number of eggs laid per female was significantly affected.Various cold-storage temperatures induced the up-regulation of the expression levels of both Hsp 70 and Hsp 90 in S.japonicum adults at 10 d after storage,and the lower the temperature,the higher the relative expression levels.The relative expression levels of Hsp 70 and Hsp 90 in the treatment group of 10℃were 7

关 键 词：日本刀角瓢虫 低温贮藏 生物学特性 存活 生长发育 热激蛋白 

分 类 号：Q968[生物学—昆虫学]