大麦籽粒纤维素、半纤维素含量全基因组关联分析  

Genome-wide association analysis of cellulose content and hemicellulose content in grains of barley

作　　者：田敏 刘新春[1] 潘佳佳 梁丽静 董雷 刘美池 冯宗云[1] TIAN Min;LIU Xin-Chun;PAN Jia-Jia;LIANG Li-Jing;DONG Lei;LIU Mei-Chi;FENG Zong-Yun(State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Research Center for Healthy Functional Crops such as Barley and Hulless Barley/College of Agronomy,Sichuan Agricultural University,Chengdu 611130,Sichuan,China)

机构地区：[1]西南作物基因资源发掘与利用国家重点实验室/四川农业大学农学院大麦青稞等健康功能作物研究中心,四川成都611130

出　　处：《作物学报》2023年第6期1726-1732,共7页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2018YFD1000705);四川省国际科技创新合作项目(2021YFH0113);财政部和农业农村部国家现代农业产业技术体系建设专项(大麦青稞,CARS-05)资助

摘　　要：纤维是衡量大麦饲用品质的重要指标之一,其遗传机制解析对饲用大麦品种选育具有一定的指导意义。本研究以316份大麦品种为材料,连续2年种植于成都、康定两地,利用分布于大麦基因组上的75,289个SNPs对籽粒纤维素、半纤维素含量进行全基因组关联分析。利用TASSEL软件的混合线性模型,纤维素、半纤维素性状分别重复检测到65个、34个显著SNPs位点(P≤0.05/N),标记的平均解释表型变异率分别为13.18%、14.10%。纤维素、半纤维素含量在3个及以上环境中重复检测到的显著相关位点分别为4个、1个,2个性状检测到了相同的SNP位点,说明2个性状存在遗传相关性。纤维素性状挖掘到6个候选基因,半纤维素性状挖掘到1个候选基因,为大麦纤维的遗传与分子机理研究及遗传改良奠定了基础。Fiber is one of the important indexes to evaluate the forage quality of barley,and the analysis of its genetic mechanism has important guiding significance for the selection and breeding of forage barley.In this study,316 barley varieties were planted in Chengdu and Kangding for two consecutive years,and 75,289 SNPs distributed in barley genome were used for genome-wide association analysis of grain cellulose and hemicellulose content,65 and 34 significant SNPs(P≤0.05/N)were repeatedly detected for cellulose and hemicellulose traits using the mixed linear model of TASSEL software,respectively.The mean explained phenotypic variation rates of markers were 13.18%and 14.10%,respectively.There were four and one significant correlation loci repeatedly detected for cellulose and hemicellulose content in three or more environments,respectively.The same SNP loci were detected for two traits,indicating that there was genetic correlation between the two traits.Six candidate genes were found for cellulose traits,and one candidate gene was found for hemicellulose traits,which laid the foundation for genetic and molecular mechanism research and genetic improvement of barley fiber.

关 键 词：大麦 全基因组关联分析 纤维素 半纤维素 

分 类 号：S512.3[农业科学—作物学]