四川乐山大佛地衣与苔藓群落中的微生物多样性  

Microbial diversity in lichen and moss habitats on Leshan Giant Buddha in Sichuan,China

作　　者：陈学萍[1,2] 白法妍 余娟 陆永生[1] 宋绍雷[2,3] 董海燕[2] 彭学艺 黄继忠 CHEN Xueping;BAI Fayan;YU Juan;LU Yongsheng;SONG Shaolei;DONG Haiyan;PENG Xueyi;HUANG Jizhong(School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China;Institute for the Conservation of Cultural Heritage,Shanghai University,Shanghai 200444,China;Nano-Science and Technology Research Center,Shanghai University,Shanghai 200444,China;Wuyou Cultural Heritage Protection Bureau of Leshan Giant Buddha,Leshan 614003,Sichuan,China)

机构地区：[1]上海大学环境与化学工程学院,上海200444 [2]上海大学文化遗产保护基础科学研究院,上海200444 [3]上海大学纳米科学与技术研究中心,上海200444 [4]乐山大佛乌尤文物保护管理局,四川乐山614003

出　　处：《上海大学学报（自然科学版）》2023年第1期166-174,共9页Journal of Shanghai University:Natural Science Edition

基　　金：国家重点研发计划资助项目(2019YFC1520500)。

摘　　要：为了探究生物侵蚀微生物的种类,通过高通量测序手段对乐山大佛地衣(lichen,LI)和苔藓样本进行了微生物(细菌、古菌和真菌)多样性分析.结果发现:地衣、活苔藓(living moss,LM)和苔藓残体(dead moss residue,DM)样本中的细菌和真菌组成在组内样本之间能进行聚类,组间差异也能良好区分;在细菌群落中,地衣与活苔藓样本中的细菌组成较为相似,两个样本的优势细菌均为蓝细菌(Cyanobacteria)和醋酸菌(Acetobacteraceae);在真菌群落中,地衣样本的优势真菌是煤炱目(Capnodiales),但其在活苔藓样本中含量较少,活苔藓样本的优势真菌是子囊菌门(Ascomycota),且其在地衣和苔藓残体样本中也有一定含量(7.47%»52.6%);古菌群落的组内差异较大,其中与氮转化相关的泉古菌(soil Crenarchaeotic group,SCG)总体丰度占比较高,并发现了较多未归类古菌(unclassified-k-norank).Microbial diversity in lichen(LI)and moss habitats on Leshan Giant Buddha was analysed using high-throughput sequencing to determine the major microorganisms responsible for the biodeterioration of this monument.Bacterial and fungal communities were clustered in the LI,live moss(LM),and dead moss residue(DM)groups.The com-munities could be distinguished among the three groups.The bacterial communities in LI and LM groups were similar,with the families Cyanobacteria and Acetobacteraceae pre-dominating.The order Capnodiales was the dominant fungal order in the LI group,but it was less abundant in the LM group.Ascomycota was the dominant fungal phylum in the LM group.The relative abundance ranged from 7.47%to 52.6%in the LI and DM groups.The archaeal community markedly differed among LI,LM,and DM groups.The soil Cre-narchaeotic group(SCG)related to nitrogen transformation was the most abundant.In addition,large numbers of unclassified-k-norank species were observed across all groups.

关 键 词：石质文物 生物侵蚀 活苔藓 地衣 苔藓残体 微生物 生物处理 

分 类 号：X172[环境科学与工程—环境科学]