麻黄多糖对PM 2.5联合卵白蛋白诱导哮喘小鼠血清代谢组学的研究  

Study on Serum Metabonomics of Ephedra Polysaccharide in Asthmatic Mice Induced by PM 2.5 Combined with Ovalbumin

作　　者：梁军[1] 王舒 苑宏宇 刘俊希 LIANG Jun;WANG Shu;YUAN Hongyu;LIU Junxi(Key Laboratory of Basic and Application Research of Beiyao,Ministry of Education,Heilongjiang Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Bases,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室,黑龙江省中药及天然药物药效物质基础重点实验室,黑龙江哈尔滨150040

出　　处：《中医药学报》2023年第4期44-49,共6页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81803809);黑龙江省博士后特别资助项目(LBH-TZ20);黑龙江省“头雁”团队支持项目(〔2019〕5号)。

摘　　要：目的:基于代谢组学方法研究麻黄多糖ESP-B4对哮喘小鼠的血清内源性代谢物的影响。方法:SPF级BALB/c雌性小鼠随机分为3组,每组6只,分别为空白组、模型组、麻黄多糖高剂量组,采用PM_(2.5)联合卵白蛋白诱导小鼠哮喘模型。采用UPLC-Q/TOF-MS方法分析比较各组小鼠的血清代谢轮廓,并对血清样品进行PCA、OPLS-DA、PLS-DA和S-plot分析,筛选出差异代谢物及相关代谢通路。结果:代谢组学共确定了22个差异代谢物,通过MetPA分析确定8条可能相关代谢通路,根据影响值的大小得出,麻黄多糖ESP-B4可显著改善小鼠血清的甘油磷脂代谢和花生四烯酸代谢水平。结论:麻黄多糖ESP-B4可能通过调节甘油磷脂代谢过程和花生四烯酸代谢过程防治哮喘,这对深入探讨其作用机制研究具有重要意义。Objective:To study the effects of Ephedra polysaccharide ESP-B4 on serum endogenous metabolites in asthmatic mice based on metabolomics.Methods:SPF BALB/c female mice were randomly divided into the blank group,the model group,and the high-dose Ephedra polysaccharide group(ESP-B4 high-dose group),with 6 mice in each group.The asthma model was induced by PM_(2.5) combined with ovalbumin in the mice.The serum metabolic profiles of mice in each group were analyzed and compared by UPLC-Q/TOF-MS,and the serum samples were analyzed by PCA,OPLS-DA,PLS-DA and S-plot to screen out the differential metabolites and related metabolic pathways.Results:A total of 22 differential metabolites were identified by metabolomics,and eight possible related metabolic pathways were identified by MetPA analysis.According to the size of the influence value,Ephedra polysaccharide ESP-B4 could significantly improve the metabolism of glycerophospholipids and arachidonic acid in the serum of mice.Conclusion:It is suggested that Ephedra polysaccharide ESP-B4 may prevent and treat asthma by regulating the metabolism of glycerophospholipid and arachidonic acid,which is of great significance for in-depth exploration of its action mechanism.

关 键 词：麻黄多糖 哮喘 PM_(2.5) 卵白蛋白 代谢组学 

分 类 号：R285.5[医药卫生—中药学]