微小RNA-574-3p在结肠癌中的表达和临床意义  

The expression and clinical significance of microRNA-574-3p in colon cancer

作　　者：孟祥彩 武玉 刘振方 王益民[1] 刘洋[1] Meng Xiangcai;Wu Yu;Liu Zhenfang;Wang Yimin;Liu Yang(Department of General Surgery,the First Hospital of Qinhuangdao,Qinhuangdao 066000,China;Department of General Surgery,Shijiazhuang Hospital of Traditional Chinese Medicine,Shijiazhuang 050051,China)

机构地区：[1]秦皇岛市第一医院普外科,秦皇岛066000 [2]石家庄市中医院普外科,石家庄050051

出　　处：《中国医师杂志》2023年第3期355-359,共5页Journal of Chinese Physician

基　　金：河北省医学科学研究课题计划(20201428)。

摘　　要：目的研究微小RNA-574-3p(miR-574-3p)在结肠癌中的表达和临床意义。方法选取2012年6月至2015年6月秦皇岛市第一医院和石家庄市中医院收治的结肠癌患者106例作为研究对象。实时荧光定量PCR(qRT-PCR)检测结肠癌组织和癌旁正常组织miR-574-3p的表达水平。分析miR-574-3p表达与结肠癌患者临床病理特征及预后的关系。免疫组织化学染色法检测miR-574-3p表达与细胞周期蛋白A2(CyclinA2)或E-钙黏蛋白(E-cadherin)表达的关系。结果相较于癌旁正常组织,106例结肠癌组织miR-574-3p表达水平显著更低(P<0.01)。miR-574-3p表达降低与肿瘤长径、Dukes分期、组织学分级及淋巴结转移有关(均P<0.05),与年龄和肿瘤位置无关(均P>0.05)。miR-574-3p表达降低、Dukes分期和组织学分级高、淋巴结转移的患者生存情况较差(均P<0.05)。癌组织miR-574-3p表达降低的患者5年总体生存率明显低于miR-574-3p表达未降低的患者(P=0.0076)。与miR-574-3p表达未降低患者相比,miR-574-3p表达降低患者结肠癌组织CyclinA2蛋白IOD值更高,E-cadherin蛋白IOD值更低(均P<0.05)。结论miR-574-3p表达降低与结肠癌患者不良预后有关,其可能通过调节CyclinA2和E-cadherin蛋白表达影响肿瘤的复发和转移。Objective To study the expression and clinical significance of microRNA-574-3p(miR-574-3p)in colon cancer.Methods A total of 106 colon cancer patients who were admitted to the First Hospital of Qinhuangdao and Shijiazhuang Hospital of Traditional Chinese Medicine from June 2012 to June 2015 were selected as the research objects.Real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression level of miR-574-3p in colon cancer tissues and normal adjacent tissues.The relationship between the expression of miR-574-3p and the clinicopathological characteristics and prognosis of patients with colon cancer was analyzed.Immunohistochemical staining was used to detect the relationship between the expression of miR-574-3p and the expression of CyclinA2 or E-cadherin.Results Compared with normal tissues adjacent to cancer,the expression level of miR-574-3p in 106 cases of colon cancer was significantly lower(P<0.01).The decreased expression of miR-574-3p was related to tumor diameter,Dukes stage,histological grade and lymph node metastasis(all P<0.05),but not to age and tumor location(all P>0.05).The patients with low expression of miR-574-3p,high Dukes stage and histological grade,and lymph node metastasis had poor survival(all P<0.05).The 5-year overall survival rate of patients with decreased miR-574-3p expression in cancer tissue was significantly lower than that of patients without decreased miR-574-3p expression(P=0.0076).Compared with patients with no decreased miR-574-3p expression,patients with decreased miR-574-3p expression had higher CyclinA2 protein integrated optical density(IOD)value and lower E-cadherin protein IOD value in colon cancer tissues(all P<0.05).Conclusions The decreased expression of miR-574-3p is related to the poor prognosis of colon cancer patients,which may affect tumor recurrence and metastasis by regulating the expression of CyclinA2 and E-cadherin proteins.

关 键 词：结肠肿瘤 miRNA-574-3p 细胞周期蛋白A2 E-钙黏蛋白 

分 类 号：R735.35[医药卫生—肿瘤]