水稻脂肪氧合酶(LOX)生物信息分析及异源表达  

Bioinformatic analysis and heterologous expression of rice lipoxygenase(LOX)

作　　者：任安然 李旭[1] 阿地兰·努热买买提 张祯 万佳欣 王婧云 杨伟程 张风丽[1] REN Anran;LI Xu;ADILAN Nuremaimaiti;ZHANG Zhen;WAN Jiaxin;WANG Jingyun;YANG Weicheng;ZHANG Fengli(School of Life Science and Technology,Shanghai Jiao Tong University,Shanghai 200240,China;School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;School of Electronic Information and Electrical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学生命科学技术学院,上海200240 [2]上海交通大学农业与生物学院,上海200240 [3]上海交通大学电子信息与电气工程学院,上海200240

出　　处：《植物生理学报》2023年第1期191-199,共9页Plant Physiology Journal

基　　金：国家自然科学基金(81973230);上海交通大学“大学生创新计划”(IPP20094)。

摘　　要：植物脂肪氧合酶(lipoxygenases, LOXs)是一类催化脂质降解并产生生理活性代谢产物的关键酶,通过脂肪氧合酶催化的一系列反应步骤为LOX途径。根据催化位置的特异性可将植物脂肪氧合酶分为2个亚家族,分别为9-LOX和13-LOX亚家族。水稻LOX-1 (OsLOX1编码)是一个非常规脂肪氧合酶,具有9-LOX和13-LOX的双重位置特异性。本研究通过对水稻脂肪氧合酶家族的生物信息分析,探究氨基酸序列相似性和保守结构域,比较双重位置特异性的关键位点;克隆水稻OsLOX1基因,发现异源表达并纯化的重组LOX具有脂肪氧合酶活性。本文对水稻脂肪氧合酶进行生物信息分析及异源表达,为揭示脂肪氧合酶双位活性机制提供基础,为双重位置特异性脂肪氧合酶在农业、食品、化工的应用奠定基础。Plant lipoxygenases(LOXs) catalyze lipid degradation and produce physiologically active metabolites. Lipoxygenases are involved in the LOX pathway which is a series of enzyme-catalyzed reactions.Plant lipoxygenases can be divided into two subfamilies, 9-LOX and 13-LOX, based on the catalytic position specificity of the substrates. The rice LOX-1(encoded by OsLOX1) is an unconventional lipoxygenase with dual position specificity. In this study, through the bioinformatics analysis of the rice lipoxygenase family, the amino acid sequence similarity and conserved domain were analyzed, and the dual position-specific conserved sites were compared. And the rice OsLOX1 gene was cloned, heterologously expressed, and purified, and recombinant LOX lipoxygenase exhibited enzyme activity. In this study, the bioinformatics analysis and heterologous expression of rice lipoxygenases provide a basis for revealing the mechanism of dual position specificity, and for applying in agriculture, food, and chemical industry.

关 键 词：水稻 脂肪氧合酶 异源表达 双重位置特异性 生物信息 

分 类 号：S511[农业科学—作物学]