响应面法优化柞蚕蛹多糖的提取工艺及其抑菌活性研究  

Optimization of the extraction process and antimicrobial activity of Tussah pupa polysaccharides by response surface methodology

作　　者：程广东[1,2] 汪梓晗 夏秋博 周清波 孙宏莱[1,2] 张春悦 夏俊 刘德江 CHENG Guangdong;WANG Zihan;XIA Qiubo;ZHOU Qingbo;SUN Honglai;ZHANG Chunyue;XIA Jun;LIU Dejiang(School of Life Sciences,Jiamusi University,Jiamusi 154007;Sino-Uzbekistan Joint Laboratory for International Cooperation in Agricultural and Forestry Technology Development and Application,Jiamusi 154007)

机构地区：[1]佳木斯大学生命科学学院,黑龙江佳木斯154007 [2]中-乌农林技术开发与应用国际合作联合实验室,黑龙江佳木斯154007

出　　处：《中国饲料》2023年第6期19-24,共6页China Feed

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(2020-KYYWF-0231)。

摘　　要：为确定柞蚕蛹多糖的最佳工艺条件及其抑菌活性,试验将柞蚕蛹作为材料,利用水提取法提取柞蚕蛹多糖,并用滤纸片法检测柞蚕蛹多糖的抑菌活性。结果表明,柞蚕蛹多糖最适宜的提取条件为料液比1∶45 g/mL,时间3.16 h,温度75℃,进行3次重复试验,所得柞蚕蛹多糖平均提取率为1.38%。柞蚕蛹多糖对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌均有抑制作用,对金黄色葡萄球菌抑制效果最好。In order to determine the best technological conditions and antibacterial activity of tussah pupa polysaccharide,the experiment took tussah pupa as material,extracted tussah pupa polysaccharide by water extraction method,and tested the antibacterial activity of tussah pupa polysaccharide by filter paper method.The results showed that the most suitable conditions for the extraction of polysaccharides from tussah pupa were the ratio of material to liquid 1:45 g/mL,time 3.16 h,temperature 75℃,and three repeated tests.The average extraction rate of polysaccharides from tussah pupa was 1.38%.Antheraea pernyi pupa polysaccharide has inhibitory effects on Staphylococcus aureus,Bacillus subtilis and Escherichia coli,and has the best inhibitory effect on Staphylococcus aureus.

关 键 词：柞蚕蛹 多糖 提取 响应面 抑菌 

分 类 号：R284.2[医药卫生—中药学]