GmSKP1,a Novel S-phase Kinase-associated Protein 1 in Glycine max,Enhancing Resistance Against Phytophthora sojae Infection  

作　　者：Ning Bin Li Wei-wei Liu Xin Ji Wei Wang Yu-hong Zhao Ming He Sheng-fu Zhang Chuan-zhong Rong Tian-yu Liu Dong-xue Xu Peng-fei Zhang Shu-zhen 

机构地区：[1]Soybean Research Institute of Northeast Agricultural University,Key Laboratory of Soybean Biology of Chinese Education Ministry,Harbin 150030,China [2]Keshan Branch Research Institute of Heilongjiang Academy of Agricultural Sciences,Keshan 161600,Heilongjiang,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2023年第1期1-12,共12页东北农业大学学报（英文版）

基　　金：Supported by the NSFC Projects(31971972);the Natural Science Foundation of Heilongjiang Province(ZD2019C001);the Outstanding Talents and Innovative Team of Agricultural Scientific Research。

摘　　要：Phytophthora root and stem rot of soybean caused by Phytophthora sojae(P.sojae)is a devastating disease that affects soybean[Glycine max(L.)Merr.]all over the world.S-phase kinase-associated protein 1(SKP1)proteins are key members of the SKP1/Cullin/F-box protein(SCF)ubiquitin ligase complex and play diverse roles in plant biology.However,the role of SKP1 in soybean against the phytopathogenic oomycete P.sojae remains unclear.In this study,a novel member of the soybean SKP1 gene family,GmSKP1 which was significantly induced by P.sojae,was reported.The expression of GmSKP1 was simultaneously induced by methyl jasmonate(MeJA),salicylic acid(SA)and ethylene(ET),which might suggest an important role for GmSKP1 of plant in responses to hormone treatments.Functional analysis using GmSKP1 overexpression lines showed that GmSKP1 enhanced resistance to P.sojae in transgenic soybean plants.Further analyses showed that GmSKP1 interacted with a homeodomain-leucine zipper protein transcription factor(GmHDL56)and a WRKY transcription factor(GmWRKY31),which could positively regulate responses to P.sojae in soybean.Importantly,several pathogenesis-related(PR)genes were constitutively activated,including GmPR1a,GmPR2,GmPR3,GmPR4,GmPR5a and GmPR10,in GmSKP1-OE soybean plants.Taken together,these results suggested that GmSKP1 enhanced resistance to P.sojae in soybean,possibly by activating the defense-related PR genes.

关 键 词：Phytophthora sojae SOYBEAN SKP1 OVEREXPRESSION pathogenesis-related gene 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]