发酵乳杆菌C6全细胞催化剂的制备及产D-塔格糖催化条件优化  

Preparation of whole-cell catalyst with Lactobacillus fermentum C6 and optimization of catalytic conditions for D-tagatose production

作　　者：邱露 彭帅英 李昆太[1,2] QIU Lu;PENG Shuaiying;LI Kuntai(School of Biological Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China;School of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China)

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045 [2]广东海洋大学食品科技学院,广东湛江524088

出　　处：《中国酿造》2023年第3期179-186,共8页China Brewing

基　　金：广东省普通高校重点领域专项(2021ZDZX4010);广东海洋大学科研启动费资助项目(060302042006)。

摘　　要：以L-阿拉伯糖异构酶产生菌发酵乳杆菌(Lactobacillus fermentum)C6为全细胞催化剂,对其发酵制备工艺以及生物转化D-塔格糖的催化反应条件和细胞透性化学处理方式进行优化。结果表明,菌株C6以最优培养基配方(葡萄糖10 g/L,酵母浸粉20 g/L,蛋白胨20 g/L,无水乙酸钠10 g/L,MgSO_(4)·7H_(2)O 0.4 g/L,MnSO_(4)·2H_(2)O 0.05 g/L,K_(2)HPO_(4)0.4 g/L,L-阿拉伯糖3 g/L,ZnSO_(4)·7H_(2)O 0.04 g/L,生物素100μg/L,焦磷酸硫胺素400μg/L)在最优发酵条件(发酵温度37℃、初始pH值7.5、装液量70 mL/150 mL、接种量1%)下培养24 h,生物量(OD_(600 nm)值=1.25)和L-阿拉伯糖异构酶酶活(107.81 U/mL)较优化前分别提高了92.31%和116.44%;全细胞催化剂在优化的催化反应条件(反应温度60℃、反应pH值7.5、Mn^(2+)10 mmol/L、底物浓度0.5 mol/L、底物加入体积2 mL,反应时间24 h)及细胞透性处理(20%丙酮处理60 min)方式下,D-塔格糖产率达45.07%。With the L-arabinose isomerase-producing strain Lactobacillus fermentum C6 as the whole-cell catalyst,the fermentation preparation process,the catalytic reaction conditions and chemical treatment of cell permeability mode for bioconversion of D-tagatose were optimized.When the strain C6 was cultured for 24 h with the optimal medium composition(glucose 10 g/L,yeast extract 20 g/L,peptone 20 g/L,anhydrous sodium acetate 10 g/L,MgSO_(4)·7H_(2)O 0.4 g/L,MnSO_(4)·2H_(2)O 0.05 g/L,K_(2)HPO_(4)0.4 g/L,L-arabinose 3 g/L,ZnSO_(4)·7H_(2)O 0.04 g/L,biotin 100μg/L,thiamine pyrophosphate 400μg/L)and fermentation condition(fermentation temperature 37℃,initial pH 7.5,liquid volume 70 ml/150 ml and inoculum 1%).The biomass(OD_(600 nm) value was 1.25)and L-arabinose isomerase activity(107.81 U/ml)of the strain C6 were increased by 92.31%and 116.44%,respectively,compared with those before optimization.Under the optimized catalytic reaction conditions(reaction temperature 60℃,reaction pH 7.5,Mn^(2+) 10 mmol/L,substrate concentration 0.5 mol/L,substrate addition volume 2 ml and reaction time 24 h)and cell permeability treatment mode(treatment with 20%acetone for 60 min),the yield of D-tagatose reached 45.07%.

关 键 词：发酵乳杆菌C6 D-塔格糖 全细胞催化剂 D-塔格糖产率 透性化细胞 

分 类 号：Q815[生物学—生物工程]