Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H_(2)O_(2)-induced oxidative damage in BRL3A cells  

作　　者：Lei Liu Yingdan Yuan Jiajia Zuo Jun Tao 

机构地区：[1]College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009,China [2]College of Horticulture,Xinyang Agriculture and Forestry University,Xinyang,Henan 464000,China [3]College of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225009,China [4]Joint International Research Laboratory of Agriculture and Agri-Product Safety,the Ministry of Education of China,Yangzhou University,Yangzhou,Jiangsu 225009,China

出　　处：《Horticultural Plant Journal》2023年第2期335-344,共10页园艺学报（英文版）

基　　金：supported by the Agricultural Scientific and Technological Independent Innovation Fund Project of Jiangsu Province[Grant No.CX(20)2030];the Modern Agricultural(Flower)Industrial Technology System Construction Project of Jiangsu Province[Grant No.JATS[2021]489].

摘　　要：Flavonoids have attracted considerable attention due to their health benefits. This study aimed to investigate the flavonoid profiles and antioxidant activity of Paeonia lactiflora petal flavonoid extract(PPF). The UHPLC-ESI-Q-Exactive HF MS/MS method was established for characterization, and 21 predominant flavonoid compounds were tentatively identified in PPF. Among them, isoscutellarein-7-(6’-acetylallosyl-(1->2)-glucoside) and scutellarin methylester were discovered in PPF for the first time. Pretreatment with PPF significantly reduced H2O2-induced cell damage, ROS accumulation, and malondialdehyde content and increased the activity of SOD, CAT, and GSH-Px in buffalo rat liver 3A(BRL3A) cells. Moreover, the expression of nuclear Factor E2-related factor(Nrf2) was upregulated by PPF, whose expression trend was consistent with that of heme oxygenase-1(HO-1), glutamate-cysteine ligase catalytic subunit(GCLC), and NAD(P)H quinone oxidoreductase-1(NQO1). These findings suggested that herbaceous peony flavonoids can be used as a natural bioactive agent to prevent oxidative stress.

关 键 词：Antioxidant activity Flavonoid Herbaceous peony petal Oxidative stress Solid-phase extraction 

分 类 号：TS201.4[轻工技术与工程—食品科学]