Recent Advances in Single-Cell Metabolomics Based on Mass Spectrometry  

作　　者：Qinlei Liu Sandra Martínez-Jarquín Renato Zenobi 

机构地区：[1]Department of Chemistry and Applied Biosciences,ETH Zurich,CH-8093 Zurich

出　　处：《CCS Chemistry》2023年第2期310-324,共15页中国化学会会刊（英文）

摘　　要：Cellular heterogeneity is essential for the physiological functions of organisms,and precise interpretation of the relevant biological mechanisms involved requires accurate measurement of biomolecules,including DNA,RNA,proteins,and metabolites at the single-cell level.Cellular metabolites react most rapidly to environmental and biochemical changes such that their detection is a critical step in determining the physiological state of cells.However,their limited amounts,structural diversity,and significant content variation render single-cell metabolomics analysis technically challenging.Mass spectrometry,with its high sensitivity,good selectivity,and wide dynamic range,has been widely used in the single-cell analysis in recent years.In terms of ionization techniques,there are four main types of single-cell mass spectrometry methods:nano-electrospray ionization mass spectrometry,matrix-assisted laser desorption/ionization mass spectrometry,secondary ion mass spectrometry,and dielectric barrier discharge ionization mass spectrometry.In this mini review,the progress of single-cellmass spectrometrymethodologies and data processing strategies are summarized,and the future trends in single-cell mass spectrometry are outlined.

关 键 词：SINGLE-CELL analysis metabolomics MASS SPECTROMETRY IONIZATION techniques ANALYTICAL methods 

分 类 号：O657.63[理学—分析化学]