miR-497-5p通过HMGA2调控食管鳞癌细胞迁移和侵袭  

miR-497-5p regulates migration and invasion of esophageal squamous cell carcinoma through HMGA2

作　　者：刘慧敏[1] 刘红红 刘晓燕 LIU Huimin;LIU Honghong;LIU Xiaoyan(Department of Gastroenterology,the Fifth Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830011,China.)

机构地区：[1]新疆医科大学第五附属医院消化科,新疆乌鲁木齐830011

出　　处：《现代肿瘤医学》2023年第8期1434-1439,共6页Journal of Modern Oncology

基　　金：新疆维吾尔自治区自然科学基金资助项目(编号:2021D01C451)。

摘　　要：目的:探讨miR-497-5p通过高迁移率族蛋白A2(HMGA2)对食管鳞癌细胞(ESCC)迁移和侵袭能力的影响。方法:利用UALCAN、GEPIA数据库分析miR-497-5p和HMGA2 mRNA在食管癌中的表达。通过生物信息学网站starBase分析miR-497-5p和HMGA2在食管癌样本中表达的相关性。通过双荧光素酶报告基因实验检测miR-497-5p对HMGA2基因的调控。采用细胞划痕实验和Transwell实验分析miR-497-5p通过HMGA2对ESCC细胞迁移和侵袭能力的影响。结果:miR-497-5p在食管癌中的表达明显低于其对照样本(P<0.05),HMGA2 mRNA在食管癌中的表达明显高于其对照样本(P<0.05)。miR-497-5p和HMGA2的表达成负相关,miR-497-5p对靶基因HMGA2具有直接调控作用。miR-497-5p mimic组细胞的迁移和侵袭能力明显低于阴性对照(NC)组(P<0.001);而miR-497-5p mimic+HMGA2组细胞的迁移和侵袭能力与miR-497-5p mimic组相比明显上升(P<0.01)。结论:miR-497-5p通过HMGA2调控食管鳞癌细胞的迁移和侵袭。Objective:To investigate the effect of miR-497-5p on the migration and invasion of esophageal squamous cell carcinoma(ESCC)by high mobility group protein A2(HMGA2).Methods:UALCAN and GEPIA databases were used to analyze the expression of miR-497-5p and HMGA2 mRNA in esophageal carcinoma(ESCA)and its control samples.The correlation between miR-497-5p and HMGA2 expression in ESCA samples was analyzed by starBase,a bioinformatics website,and the binding sites between them were predicted.The regulation effect of miR-497-5p on HMGA2 was confirmed by dual luciferase reporter assay.The effect of miR-497-5p on the migration and invasion of ESCC cells through HMGA2 was analyzed by Wound healing assay and Transwell assay.Results:The expression of miR-497-5p in ESCA was significantly lower than that in the control samples(P<0.05),and the expression of HMGA2 mRNA in ESCA was significantly higher than that in the control samples(P<0.05).miR-497-5p was negatively correlated with HMGA2 in ESCA samples.There were binding sites between miR-497-5p and HMGA2,and miR-497-5p directly regulated the target gene HMGA2.The migration and invasion ability of miR-497-5p mimic group were significantly lower than that of negative control(NC)group(P<0.001).The migration and invasion ability of miR-497-5p mimic+HMGA2 group were significantly increased compared with mir-497-5p mimic group(P<0.01).Conclusion:miR-497-5p regulates the migration and invasion of ESCC via HMGA2.

关 键 词：miR-497-5p HMGA2 食管鳞癌(ESCC) 迁移和侵袭 

分 类 号：R735.1[医药卫生—肿瘤]