基于CD19/IgM亚群分析套细胞淋巴瘤骨髓中T细胞的构比异常  

The expression characteristics of T cells in mantle cell lymphoma bone marrow based on CD19/IgM sub-populations

作　　者：胡雯博 唐君玲 李晓明 HU Wenbo;TANG Junling;LI Xiaoming(Department of Hematology,the Affiliated Hospital of Southwest Medical University,Sichuan Luzhou 646000,China.)

机构地区：[1]西南医科大学附属医院血液内科,四川泸州646000

出　　处：《现代肿瘤医学》2023年第8期1481-1485,共5页Journal of Modern Oncology

基　　金：泸州市科技局应用基础研究项目(编号:2019LZXNYDJ54);四川省重点研发项目(编号:2019YFS0301)。

摘　　要：目的:研究T细胞在初诊IV期套细胞淋巴瘤(mantle cell lymphoma,MCL)骨髓单个核细胞中的差异表达。方法:首先流式检测健康供者及MCL组中CD19-/IgM-,CD19-/IgM+,CD19+/IgM-,CD19+/IgM+亚群的比例;其次检测CD45+CD3+T细胞在两组中的总比例;最后检测T细胞在两组四个亚群中的占比。结果:四个亚群在组内有极其显著的差异(P<0.0001),两组间无统计学差异。T细胞在MCL组占(0.46±0.13)%较健康供者组(1.10±0.06)%显著减少(P=0.0251)。四个亚群中T细胞占比在健康供者组内有极其显著的差异(P<0.0001),而MCL组内差异消失(P=0.3294)。T细胞在MCL组CD19-/IgM-亚组中占(0.02±0.01)%较健康供者组(1.51±0.35)%下降(P<0.0001)。CD19+/IgM-亚组中T细胞在健康供者组、MCL组占比分别为(4.28±0.14)%、(0.65±0.45)%,MCL组仍然呈现非常显著的下降(P=0.0012),其余两个亚组在两组间比较无统计学差异。结论:与健康供者比较初诊IV期MCL骨髓病灶中T细胞总占比明显减少;在CD19-/IgM-、CD19+/IgM-亚组中T细胞占比显著减少,提示MCL骨髓病灶中缺乏正常数量T细胞的免疫监控作用,从而可能促进肿瘤的发生发展。Objective:To investigate the differential expression of T cells in bone marrow mononuclear cells of newly diagnosed stage IV mantle cell lymphoma(MCL).Methods:Firstly,the proportions of CD19-/IgM-,CD19-/IgM+,CD19+/IgM-,CD19+/IgM+sub-populations in healthy donors and MCL group were detected by flow cytometry.Secondly,the total proportion of CD45+CD3+T cells in the two groups was detected.Finally,the ratios of T cells in the four sub-populations of the two groups were detected.Results:There were significant differences among the four sub-populations in each group(P<0.0001),and there was no significant difference between the two groups.The percentage of T cells in MCL group(0.46±0.13)%was significantly lower than that in healthy donor group(1.10±0.06)%(P=0.0251).There was a significant difference in the proportion of T cells among the four sub-populations in the healthy donors group(P<0.0001),but such difference wasn't found in the MCL group(P=0.3294).The percentage of T cells in the sub-populations of CD19-/IgM-in the MCL group was(0.02±0.01)%,which was lower than that of the healthy donor group(1.51±0.35)%(P<0.0001).The percentage of T cells in the sub-population of CD19+/IgM-in healthy donor group and MCL group was(4.28±0.14)%and(0.65±0.45)%,respectively.The ratio of T cells in the sub-population of CD19+/IgM-in MCL group was still significantly decreased(P=0.0012).There was no statistical difference for the other two subgroups between the two groups.Conclusion:Compared with healthy donors,the total proportion of T cells in bone marrow lesions of newly diagnosed MCL with stage IV was significantly decreased and the percentages of T cells in the sub-populations of CD19-/IgM-and CD19+/IgM-were significantly decreased.It is suggested that the lack of normal number of T cells in the bone marrow lesions of MCL,and leads to weakened immune monitoring,which may promote the occurrence and development of tumors.

关 键 词：T细胞 CD19/IgM亚群 套细胞淋巴瘤 

分 类 号：R733.4[医药卫生—肿瘤]