FBXL2和Dkk-1在结直肠癌组织中的表达及临床意义  

Expression and clinical significance of FBXL2 and Dkk-1 in colorectal cancer

作　　者：李晓宁[1] 李小龙 宋瑞[2] 张惠卿[3] Li Xiaoning;Li Xiaolong;Song Rui;Zhang Huiqing(The Second Department of Surgery,Baoding First Central Hospital,Hebei Province,Baoding 071000,China;不详)

机构地区：[1]河北省保定市第一中心医院普通外二科,071000 [2]河北省保定市第一中心医院普通外一科,071000 [3]河北省保定市第一中心医院普通外三科,071000

出　　处：《疑难病杂志》2023年第4期377-382,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省医学科学研究课题(20191111);保定市社发类项目(18ZF262)。

摘　　要：目的研究结直肠癌(CRC)组织中F-盒富含亮氨酸的重复蛋白2(FBXL2)、Wnt信号通路抑制剂1(Dkk-1)的表达及临床意义。方法选取2017年10月—2019年5月保定市第一中心医院普通外科诊治CRC患者75例作为研究对象。应用荧光定量PCR及免疫组化检测CRC癌组织和癌旁组织中FBXL2、Dkk-1 mRNA及蛋白表达水平。比较不同临床病理特征CRC患者癌组织中FBXL2、Dkk-1蛋白表达差异。Kaplan-Meier生存分析(Log-rank检验)FBXL2、Dkk-1蛋白表达与CRC患者生存预后的关系。多因素COX回归分析影响CRC患者生存预后的危险因素。结果CRC癌组织中FBXL2、Dkk-1 mRNA表达水平低于癌旁组织(t=25.053、34.053,P均<0.001)。CRC癌组织中FBXL2、Dkk-1蛋白表达阳性率低于癌旁组织(χ^(2)=58.134、51.042,P均<0.001)。CRC癌组织中FBXL2与Dkk-1蛋白表达呈显著正相关(r s=0.714,P<0.001)。TNM分期Ⅰ~Ⅱ期、淋巴结转移阴性CRC患者癌组织中FBXL2、Dkk-1蛋白表达阳性率高于TNM分期Ⅲ期、淋巴结转移阳性患者,差异均有统计学意义(χ^(2)/P=7.588/0.006、5.220/0.022)。FBXL2阴性表达组患者3年累积生存率明显低于阳性表达组患者(χ^(2)=5.991,P=0.014);Dkk-1阴性表达组患者3年累积生存率明显低于阳性表达组患者(χ^(2)=8.058,P=0.005)。肿瘤分期Ⅲ期、淋巴结转移阳性、FBXL2阴性表达、Dkk-1阴性表达是影响CRC患者预后的独立危险因素[HR(95%CI)=1.613(1.223~2.126),1.917(1.314~2.799),1.837(1.229~2.745),1.738(1.246~2.426),P均<0.001]。结论CRC癌组织中FBXL2、Dkk-1表达降低,两者表达与CRC肿瘤分期及淋巴结转移有关,是评估CRC患者生存预后新的肿瘤标志物。Objective To study the expression and clinical significance of F-box leucine-rich repeat protein 2(FBXL2)and Wnt signal pathway inhibitor 1(Dkk-1)in colorectal cancer(CRC).Methods From October 2017 to May 2019,75 patients with CRC diagnosed and treated by general surgery in Baoding First Central Hospital were selected as the study subjects.The expression levels of FBXL2,Dkk-1 mRNA and protein in CRC carcinoma and adjacent tissues were detected by fluorescence quantitative PCR and immunohistochemistry.To compare the differences of FBXL2 and Dkk-1 protein expression in cancer tissues of CRC patients with different clinicopathological characteristics.Kaplan-Meier survival analysis(Log-rank test)The relationship between the expression of FBXL2 and Dkk-1 protein and the survival and prognosis of CRC patients.Multivariate COX regression analysis was used to analyze the risk factors affecting the survival and prognosis of CRC patients.Results The expression level of FBXL2 and Dkk-1 mRNA in CRC cancer tissue was lower than that in adjacent tissues(t=25.053,34.053,P<0.001).The positive rate of FBXL2 and Dkk-1 protein expression in CRC cancer tissue is lower than that in adjacent tissues(χ^(2)=58.134,51.042,P<0.001).There was a significant positive correlation between FBXL2 and Dkk-1 protein expression in CRC cancer tissue(rs=0.714,P<0.001).The positive rates of FBXL2 and Dkk-1 protein expression in cancer tissues of patients with stageⅠto stageⅡof TNM stage and negative lymph node metastasis were higher than those of patients with stageⅢof TNM stage and positive lymph node metastasis.The difference was statistically significant(χ^(2)/P=7.588/0.006,5.220/0.022).The 3-year cumulative survival rate of patients with negative FBXL2 expression was significantly lower than that of patients with positive FBXL2 expression(χ^(2)=5.991,P=0.014).The 3-year cumulative survival rate of DKK-1 negative expression group was significantly lower than that of positive expression group(χ^(2)=8.058,P=0.005).Tumor stage III,positive l

关 键 词：结直肠癌 F-盒富含亮氨酸的重复蛋白2 Wnt信号通路抑制剂1 临床预后 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]