肝癌中线粒体膜蛋白ATAD3A表达与临床病理特征及预后的关系  

Relationship between mitochondrial membrane protein ATAD3A expression and clinicopathological featuresandprognosis inhepatocellularcarcinoma

作　　者：李晖[1] 范志勇[1] 耿西林[1] 常虎林[1] 吴武军[1] 张煜[1] Li Hui;Fan Zhiyong;Geng Xilin;Chang Hulin;Wu Wujun;Zhang Yu(Liver and Gallbladder Surgery,Shaanxi Provincial People's Hospital,Xi'an Shaanxi Province 710068,China)

机构地区：[1]陕西省人民医院肝胆外科,西安710068

出　　处：《中华普外科手术学杂志（电子版）》2023年第2期157-161,共5页Chinese Journal of Operative Procedures of General Surgery(Electronic Edition)

基　　金：陕西省自然科学基础研究计划(2020JQ-948);陕西省科技研究发展(攻关)计划项目(2014K11-03-03-11)。

摘　　要：目的:检测肝癌组织中线粒体膜蛋白ATAD3A表达情况,探究其与肝癌临床病理特征及预后的关系。方法:选取2016年7月至2018年3月行手术治疗的85例肝癌患者癌组织及其对应癌旁组织(距癌灶边缘3~5 cm)标本进行研究。采用免疫组织化学染色法和qRT-PCR检测肝癌组织及癌旁组织中ATAD3A蛋白及mRNA表达情况。采用SPSS 25.0统计软件进行数据分析,计量资料以(xˉ±s)表示,计数资料用[n(%)]表示,ATAD3A表达与临床病理特征的相关性采用χ^(2)或t检验,Kaplan-Meier生存曲线分析预后,COX回归分析预后生存危险因素。P<0.05认为差异有统计学意义。结果:肝癌组织中ATAD3A蛋白阳性表达率为28.2%(24/85),mRNA相对表达水平为(2.6±0.5),明显低于癌旁正常组织的76.5%(65/85)和(4.8±0.9),差异有统计学意义(χ^(2)=39.641,t=19.701,P<0.001)。ATAD3A蛋白及mRNA表达与肝癌肿瘤直径、分化程度、TNM分期及脉管癌栓等病理特征关系密切,差异有统计学意义(χ^(2)=39.641,P<0.05)。ATAD3A阳性组患者术后5年累积总生存率为58.3%,阴性组患者术后5年累积总生存率为41.0%,差异有统计学意义(Log-Rank χ^(2)=4.317,P=0.038)。肿瘤直径、分化程度、TNM分期、伴脉管癌栓及ATAD3A阴性表达是影响肝癌患者预后不良的独立危险因素(P<0.05)。结论:肝癌组织中ATAD3A显著阴性/低表达,与肿瘤直径、分化程度、TNM分期、脉管癌栓等病理特征关系密切,可能参与病情进展;ATAD3A阴性表达是影响肝癌患者预后不良的独立危险因素,可将其作为判断预后的分子标志物。Objective The expression of mitochondrial membrane protein ATAD3A in liver cancer tissues was detected to explore its relationship with the clinicopathologic features and prognosis of liver cancer.Methods Cancer tissues and corresponding paracancer tissues(3~5 cm away from the edge of cancer foci)of 85 patients with liver cancer who underwent surgical treatment from July 2016 to March 2018 were selected for study.Immunohistochemical staining and qRT-PCR were used to detect the expression of ATAD3A protein and mRNA in liver cancer tissues and adjacent tissues.SPSS 25.O statistical software was used for data analysis,the measurement data were represented by(x+s),and the count data were represented by[n(%)].The correlation between ATAD3A expression and clinicopathological features was analyzed by X or t test,Kaplan-Meier survival curve was used to analyze the prognosis,and Cox regression was used to analyze the prognosis and survival risk factors.P<0.05 was considered statistically significant.ResultsThe positive expression rate of ATAD3A protein in liver cancer tissues was 28.2%(24/85),and the relative expression level of mRNA was(2.6+0.5),which was significantly lower than that of adjacent normal tissues 76.5%(65/85)and(4.8±0.9),and the difference was statistically significant(χ^(2)=39.641,t=19.701,P<0.001).The expression of ATAD3A protein and mRNA were closely related to tumor diameter,differentiation degree,TNM stage and vascular cancer thrombus,and the differences were statistically significant(x=39.641,P<0.05).The 5-year cumulative overall survival rate was 58.3%in the ATAD3A positive group and 41.0%in the negative group,with statistically significant dfferences(Log-Rank χ^(2)=4.317,P=0.038).Tumor diameter,degree of differentiation,TNM stage,vascular cancer thrombin and ATAD3A negative expression were independent risk factors for poor prognosis in patients with HCC(P<0.05).Conclusion ATAD3A expression was significantly negative/low in liver cancer tissues,which was closely related to tumor diameter,differen

关 键 词：癌 肝细胞 线粒体膜转运蛋白质类 三磷酸腺苷酶家族蛋白3A 病理学 临床 预后 

分 类 号：R735.7[医药卫生—肿瘤]