有机酸分析柱在饲料酸化剂质量检测中的应用研究  

Study on the Application of Organic Acid Analysis Column in the Quality Detection of Feed Acidifier

作　　者：张天姝 杨希国 陈玉艳 李丽 刘石鑫 ZHANG Tianshu;YANG Xiguo;CHEN Yuyan;LI Li;LIU Shixin(Liaoning Inspection,Examination&Certification Centre,Shenyang 110036,China;Liaoning General Fair Testing Co.,Ltd.,Shenyang 110026,China)

机构地区：[1]辽宁省检验检测认证中心,辽宁沈阳110036 [2]辽宁通正检测有限公司,辽宁沈阳110026

出　　处：《品牌与标准化》2023年第2期90-92,96,共4页

摘　　要：建立一种采用Aminex HPX-87H有机酸分析柱分离,高效液相色谱法同步检测饲料酸化剂中甲酸、乙酸、丙酸、丁酸、乳酸、柠檬酸、苹果酸含量的方法。样品采用水超声提取,应用Aminex?HPX-87H有机酸分析柱,以0.01 mol/L硫酸溶液为流动相,二极管阵列检测器在210 nm进行定量检测。结果表明:有机酸标准溶液线性关系良好,相关系数均大于0.995,回收率均大于90.0%,变异系数小于10%。该方法具有专属性强、准确度高的特点。To establish an Aminex HEPX-87H organic acid analytical column for the simultaneous determination of formic acid,acetic acid,propionic acid,butyric acid,lactic acid,citric acid and malic acid in feed acidifiers by HPLC.The samples were extracted by ultrasonic water.Aminex?HEPX-87H organic acid analysis column was used for the quantitative detection at 210 nm with 0.01 mol/L sulfuric acid solution as the mobile phase and a diode array detector.The results showed that the linear relationship of organic acid standard solution was good,the correlation coefficients were all greater than 0.995,the recoveries were all greater than 90.0%,and the coefficient of variation was less than 10%.The method has the characteristics of strong specificity and high accuracy.

关 键 词：高效液相色谱法 酸化剂 有机酸 

分 类 号：S816.17[农业科学—饲料科学] O657.72[农业科学—畜牧学]