毛兰素对人肝癌细胞系HEPG2增殖、迁移、凋亡和上皮间质转化的影响及其机制  

Effects and mechanism of Erianin on proliferation,migration,apoptosis and epithelial-mesenchymal transition of human hepatoma cell line

作　　者：虞璐 张怡莹 叶佳琪[1] 杨克敏[3] YU Lu;ZHANG Yiying;YE Jiaqi;YANG Kemin(Department of Hepatology,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200040,China;不详)

机构地区：[1]上海中医药大学附属龙华医院肝病科,上海200040 [2]上海市中医医院药剂科 [3]上海市静安区中心医院中医科

出　　处：《山东医药》2023年第9期17-21,共5页Shandong Medical Journal

摘　　要：目的观察毛兰素对人肝癌细胞系HEPG2增殖、迁移、凋亡和上皮间质转化的影响,并探讨其机制。方法取对数生长期HEPG2细胞分为对照组、毛兰素组、毛兰素+pc-DNA组、毛兰素+pc-Snail组:对照组仅为HEPG2细胞,毛兰素组加入40 nmol/L的毛兰素,毛兰素+pc-DNA组转染空白对照质粒pc-DNA并加入40 nmol/L的毛兰素,毛兰素+pc-Snail组转染Snail过表达质粒pc-Snail并加入40 nmol/L的毛兰素。各组细胞继续培养24 h后,采用平板克隆法观察各组细胞增殖能力,以细胞克隆个数表示;采用Transwell小室法观察各组细胞迁移能力,以细胞迁移个数表示;采用流式细胞术检测各组细胞凋亡率;采用Western blotting法检测各组细胞Snail蛋白、凋亡相关蛋白(Bax、Bcl-2)、间质转化相关蛋白(E-cadherin、N-cadherin、Vimentin)。结果对照组、毛兰素组、毛兰素+pc-DNA组、毛兰素+pc-Snail组细胞克隆个数分别为(109.17±6.98)、(47.50±5.04)、(41.67±4.92)、(82.33±6.25)个,细胞迁移个数分别为(171.33±8.36)、(115.17±7.94)、(108.83±7.68)、(143.17±8.45)个,细胞凋亡率分别为1.83%±0.22%、29.57%±2.34%、32.79%±2.38%、16.16%±2.05%,其中毛兰素组和毛兰素+pc-DNA组细胞克隆个数、细胞迁移个数、细胞凋亡率无差异(P>0.05),其余组间细胞克隆个数、细胞迁移个数、细胞凋亡率相比,P均<0.05。与对照组相比,毛兰素组、毛兰素+pc-DNA组、毛兰素+pc-Snail组Snail、Bcl-2、N-cadherin、Vimentin蛋白相对表达量均降低(P均<0.05),Bax、E-cadherin蛋白相对表达量均升高(P均<0.05);与毛兰素组和毛兰素+pc-DNA组相比,毛兰素+pc-Snail组Snail、Bcl-2、N-cadherin、Vimentin蛋白相对表达量均升高(P均<0.05),Bax、E-cadherin蛋白相对表达量均降低(P均<0.05)。结论毛兰素可抑制HEPG2细胞的增殖、迁移和上皮间质转化,促进细胞凋亡,其机制与毛兰素可抑制Snail信号通路相关蛋白的表达有关。Objective To observe the effects of Erianin on the proliferation,migration,apoptosis and epithelial-mesenchymal transition(EMT)of human hepatoma cell line HEPG2,and to explore their mechanism.Methods HEPG2 cells in the logarithmic growth phase were divided into the control group,Erianin group,Erianin+pc-DNA group,and Erianin+pc-Snail group:the control group was only HEPG2 cells,cells in the Erianin group were added with 40 nmol/L Erianin,cells in the Erianin+pc-DNA group were transfected with blank control plasmid pc-DNA and then were added with 40 nmol/L Erianin,cells in the Erianin+pc-Snail group were transfected with Snail overexpression plasmid pc-Snail and were added with 40 nmol/L Erianin.After the cells in each group continued to be cultured for 24 h,the proliferative ability of cells in each group was observed by plate cloning method,expressed by the number of cell clones;Transwell chamber method was applied to observe the migration ability of cells in each group,expressed by the number of cell migration;the apoptosis rate was detected by flow cytometry;the Snail protein,apoptosis-related proteins(Bax,Bcl-2),and EMT-related proteins(E-cadherin,N-cadherin,Vimentin)were detected by Western blotting.Results The numbers of cell clones in the control group,Erianin group,Erianin+pc-DNA group and Erianin+pc-Snail group were 109.17±6.98,47.50±5.04,41.67±4.92 and 82.33±6.25,respectively;the numbers of migration cells were 171.33±8.36,115.17±7.94,108.83±7.68 and 143.17±8.45,respectively;the apoptosis rates were 1.83%±0.22%,29.57%±2.34%,32.79%±2.38%,and 16.16%±2.05%,respectively,there were no difference in the number of cell clones,the number of cell migration,or the rate of apoptosis between the Erianin group and the Erianin+pc-DNA group(all P>0.05),while significant differences were found in the number of cell clones,the number of cell migration and the rate of apoptosis among the other groups(all P<0.05).Compared with the normal group,the relative expression levels of Snail,Bcl-2,N-cadherin and Viment

关 键 词：毛兰素 肝癌 细胞增殖 细胞迁移 细胞凋亡 上皮间质转化 Snail信号通路 

分 类 号：R735.7[医药卫生—肿瘤]