食管鳞癌组织中VEGFR-2基因扩增情况观察及与患者预后关系分析  

VEGFR-2 gene amplification in esophageal squamous cell carcinoma and its relationship with prognosis

作　　者：吐尔洪·托合提 阿丽米热·库尔班 卡吾力·居买 居来提·艾尼瓦尔[1] 张海平[1] 伊地力斯·阿吾提[1] Turgon Tohti;Alimire Kurban;Kawul Juma;Jurat Anwar;ZHANG Haiping;Edris Awut(The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;不详)

机构地区：[1]新疆医科大学第一附属医院,乌鲁木齐830054 [2]新疆医科大学基础医学院

出　　处：《山东医药》2023年第9期31-35,共5页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81960498)。

摘　　要：目的观察食管鳞癌组织中血管内皮生长因子受体-2(VEGFR-2)基因扩增情况,并分析VEGFR-2基因扩增与食管鳞癌患者预后的关系。方法食管鳞癌患者120例,术中留取食管癌组织,采用荧光原位杂交(FISH)法观察食管鳞癌组织中VEGFR-2基因扩增情况,并分析VEGFR-2基因扩增与食管鳞癌患者临床病理参数的关系。根据有无VEGFR-2基因扩增,将120例食管鳞癌患者分为阳性组(VEGFR-2基因扩增阳性)和阴性组(VEGFR-2基因扩增阴性),采用Kaplan-Meier生存分析法及多因素COX回归模型分析VEGFR-2基因扩增与食管鳞癌患者预后的关系。结果120例食管鳞癌患者中,49例患者存在VEGFR-2基因扩增,VEGFR-2基因扩增率为40.8%。VEGFR-2基因扩增与食管鳞癌患者肿瘤分化程度、有无淋巴结转移、有无脉管浸润有关(P均<0.05)。阴性组的食管鳞癌患者生存时间为16(11.15~20.84)个月,阳性组为13(8.27~17.72)个月,两组相比,P<0.05。多因素COX回归分析结果显示,VEGFR-2基因扩增是食管鳞癌患者不良预后的独立危险因素(HR=0.562,95%CI:0.374~0.844,P=0.006)。结论部分食管鳞癌患者存在VEGFR-2基因扩增。VEGFR-2基因扩增与食管鳞癌肿瘤分化程度、有无淋巴结转移、有无脉管浸润有关,是食管鳞癌患者不良预后的独立危险因素。Objective To observe the amplification of vascular endothelial growth factor receptor-2(VEGFR-2)gene in esophageal squamous cell carcinoma,and to analyze the relationship between the amplification of VEGFR-2 gene and the prognosis of esophageal squamous cell carcinoma patients.Methods In 120 patients with esophageal squamous cell carcinoma,esophageal cancer tissues were taken during operation.The amplification of VEGFR-2 gene in esophageal squamous cell carcinoma tissue was observed by fluorescence in situ hybridization(FISH),and the relationships between the amplification of VEGFR-2 gene and the clinicopathologic parameters of esophageal squamous cell carcinoma patients were analyzed.According to the presence or absence of VEGFR-2 gene amplification,120 patients with esophageal squamous cell carcinoma were divided into the positive group(VEGFR-2 gene amplification positive)and negative group(VEGFR-2 gene amplification negative).The relationship between VEGFR-2 gene amplification and prognosis of patients with esophageal squamous cell carcinoma was analyzed by Kaplan-Meier survival analysis and multivariate COX regression model.Results Among 120 patients with esophageal squamous cell carcinoma,49 patients had VEGFR-2 gene amplification,and the amplification rate of VEGFR-2 gene was 40.8%.The amplification of VEGFR-2 gene was related to the degree of tumor differentiation,lymph node metastasis and vascular invasion in patients with esophageal squamous cell carcinoma(all P<0.05).The survival time of esophageal squamous cell carcinoma patients in the negative group was 16(11.15-20.84)months,and that in the positive group was 13(8.27-17.72)months,with statistically significant difference(P<0.05).Multivariate COX regression analysis showed that VEGFR-2 gene amplification was an independent risk factor for poor prognosis in patients with esophageal squamous cell carcinoma(HR=0.562,95%CI:0.374-0.844,P=0.006).Conclusion Some patients with esophageal squamous cell carcinoma have VEGFR-2 gene amplification.The amplificatio

关 键 词：血管内皮生长因子受体-2基因 基因扩增 食管癌 食管鳞状细胞癌 

分 类 号：R[医药卫生]