呼吸睡眠暂停综合征与特发性肺纤维化的共表达差异基因分析  

Analysis of co-expressed differential genes between sleep apnea syndrome and idiopathic pulmonary fibrosis

作　　者：陈铃 CHEN Ling(The Second People's Hospital of Lishui City,Zhejiang 323000,China)

机构地区：[1]丽水市第二人民医院,浙江323000

出　　处：《中国卫生检验杂志》2023年第5期607-611,共5页Chinese Journal of Health Laboratory Technology

摘　　要：目的 通过生物信息学方法寻找呼吸睡眠暂停综合征(OSA)与特发性肺纤维化(IPF)之间的共表达差异基因。方法 下载GSE24206和GSE135917数据集作为研究对象,筛选疾病组与正常对照组之间的差异表达基因(DEG),运用韦恩工具获得彼此的共同表达DEG(co-DEGs)。运用WebGestalt数据库对co-DEGs进行GO/KEGG分析,通过STRING数据库分析co-DEGs的蛋白互作网络以及相关枢纽基因,使用pROC包对枢纽基因在OSA和IPF中的诊断价值进行ROC分析。结果 共筛选出406个co-DEGs, GO/KEGG富集分析显示co-DEGs在生物过程中主要在细胞外基质组织,细胞外结构组织,白细胞迁移,免疫系统过程的负调节,对细菌来源分子的反应,中性粒细胞介导的免疫等过程中特别富集。在细胞组成中主要富集在含胶原的细胞外基质、轴丝部分、特定颗粒、基底膜内,而对于分子功能主要在受体配体活性、糖胺聚糖结合、DNA结合转录激活因子活性、RNA聚合酶Ⅱ特异性、硫化合物结合、G蛋白偶联受体结合、细胞外基质结构成分、细胞因子活性等功能上发生富集。对于通路分析,co-DEGs主要富集在IL-17信号通路,病毒蛋白与细胞因子和细胞因子受体的相互作用,TNF信号通路、类风湿关节炎、细胞因子-细胞因子受体相互作用,ECM-受体相互作用等信号通路。通过STRING数据库的互作网络分析筛选出的前5个枢纽基因(IL-6、CXCL8、PTGS2、FOS、MYC)在诊断OSA和IPF上具有一定的准确性。结论 OSA和IPF之间存在co-DEGs,彼此之间通过多种信号通路在疾病进展中发挥作用。Objective This paper aims to find the co-expressed differential genes between sleep apnea syndrome(OSA) and idiopathic pulmonary fibrosis(IPF) through bioinformatics methods. Methods GSE24206 and GSE135917 data sets were download as the research objects, the differentially expressed genes(DEG) between the disease group and the control group were screened, and Wayne tool was used to obtain each other’s co-expressed DEGs(co-DEGs). WebGestalt database was used to perform GO/KEGG analysis on co-DEGs. The protein interaction network and related hub genes of co-DEGs were analyzed by STRING database, and the diagnostic value of hub genes in OSA and IPF was analyzed by ROC with pROC package. Results A total of 406 co-DEGs were screened. GO/KEGG enrichment analysis showed that co-DEGs are especially enriched in extracellular matrix tissues, extracellular structural tissues, leukocyte migration, negative regulation of immune system processes, response to bacterial-derived molecules, and neutrogranulocyte mediated immunity. In the cell composition, it is mainly enriched in the extracellular matrix containing collagen, the axial filament part, specific particles, and the basement membrane. The molecular functions are mainly enriched in receptor ligand activity, glycosaminoglycan binding, DNA binding transcriptional activator activity, RNA polymerase Ⅱ specificity, sulfur compound binding, G protein-coupled receptor binding, extracellular matrix structural components, cytokine activity and other functions. For pathway analysis, co-DEGs were mainly concentrated in IL-17 signaling pathway, interaction between viral proteins and cytokines and cytokine receptors, TNF signaling pathway, rheumatoid arthritis, cytokine-cytokine receptor interaction, ECM-receptor interaction and other signaling pathways. The first 5 hub genes(IL-6, CXCL8, PTGS2, FOS, MYC) screened by the interaction network analysis of STRING database showed certain accuracy in the diagnosis of OSA and IPF. Conclusion co-DEGs exist between OSA and IPF and play a

关 键 词：呼吸睡眠暂停综合征 特发性肺纤维化 共表达差异基因 生物信息学方法 

分 类 号：R563.8[医药卫生—呼吸系统]