Altered gene expression in human brain microvascular endothelial cells in response to the infection of influenza H1N1 virus  

作　　者：Doaa Higazy Xianwu Lin Tanghui Xie Ke Wang Xiaochen Gao Min Cui 

机构地区：[1]Microbiology Department,Faculty of Agriculture,Cairo University,Giza 12613,Egypt [2]Key Laboratory of Preventive Veterinary Medicine in Hubei Province,The Cooperative Innovation Center for Sustainable Pig Production,Wuhan 430070,Hubei,China [3]Key Laboratory of Development of Veterinary Diagnostic Products,Ministry of Agriculture of the People’s Republic of China,Wuhan 430070,Hubei,China [4]International Research Center for Animal Disease,Ministry of Science and Technology of the People’s Republic of China,Wuhan 430070,Hubei,China [5]State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,No.1 Shizishan St.Huazhong Agricultural University,Wuhan 430070,Hubei,China [6]College of Informatics,Huazhong Agricultural University,Wuhan 430070,Hubei,China

出　　处：《Animal Diseases》2023年第1期1-20,共20页动物疾病（英文）

基　　金：the financial support provided by the National Program on Key Research Project of China(2016YFD0500406);the National Natural Sciences Foundation of China(Grant No.31872455);the Fundamental Research Funds for the Central Universities(2662018PY016);the Start-up Research Fund from Huazhong Agricultural University.

摘　　要：Influenza viruses not only cause respiratory illness,but also have been reported to elicit neurological manifestations following acute viral infection.The central nervous system(CNS)has a specific defense mechanism against pathogens structured by cerebral microvasculature lined with brain endothelial cells to form the blood–brain barrier(BBB).To investigate the response of human brain microvascular endothelial cells(hBMECs)to the Influenza A virus(IAV),we inoculated the cells with the A/WSN/33(H1N1)virus.We then conducted an RNAseq experiment to determine the changes in gene expression levels and the activated disease pathways following infection.The analysis revealed an effective activation of the innate immune defense by inducing the pattern recognition receptors(PRRs).Along with the production of proinflammatory cytokines,we detected an upregulation of interferons and interferon-stimulated genes,such as IFN-β/λ,ISG15,CXCL11,CXCL3 and IL-6,etc.Moreover,infected hBMECs exhibited a disruption in the cytoskeletal structure both on the transcriptomic and cytological levels.The RNAseq analysis showed different pathways and candidate genes associated with the neuroactive ligand-receptor interaction,neuroinflammation,and neurodegenerative diseases,together with a predicted activation of the neuroglia.Likewise,some genes linked with the mitochondrial structure and function displayed a significantly altered expression.En masse,this data supports that hBMECs could be infected by the IAV,which induces the innate and inflammatory immune response.The results suggest that the influenza virus infection could potentially induce a subsequent aggravation of neurological disorders.

关 键 词：Blood-brain barrier Influenza A virus(IAV) hBMECs CNS Neurodegenerative diseases RNASEQ 

分 类 号：S85[农业科学—兽医学]