幼羊硬脑膜mRNA表达差异及影响颅缝处细胞迁移的信号通路探究  

Analysis on the differential expression of mRNA in the dura mater of Ovis aries and the potential signaling pathways affecting cell migration

作　　者：王孟辉 高岩[1,2,3,4] 叶小健[1,2,3,4] 张春阳 邵国 吴启润[1] Wang Menghui;GaoYan;Ye Xiaojian;Zhang Chunyang;Shao Guo;Wu Qirun(Baotou Medical College,Baotou 014010,Inner Mongolia Province,China;Department of Neurosurgery,the First Affiliated Hospital of Baotou Medical College,Baotou 014010,Inner Mongolia Province,China;Institute of Neurosurgical Diseases of Baotou Medical College(Translational?medicine),Baotou 014010,Inner Mongolia Province,China;Engineering Technology Center for Bone Tissue Regeneration and Injury Repair in Inner Mongolia autonomous region,Baotou 014010,Inner Mongolia Province,China;Transformation Medical Center of Longgang Third People's Hospital,Shenzhen 518100,Guangdong Province,China)

机构地区：[1]内蒙古科技大学包头医学院第一附属医院,内蒙古包头014040 [2]内蒙古科技大学包头医学院第一附属医院神经外科,内蒙古包头014010 [3]包头医学院神经外科疾病研究所(转化医学),内蒙古包头014010 [4]内蒙古自治区骨组织再生与损伤修复工程技术中心,内蒙古包头014010 [5]深圳市龙岗区第三人民医院转化医学中心,广东深圳518100

出　　处：《中国临床解剖学杂志》2023年第2期187-193,共7页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(No.81960238,82160250);内蒙古科技厅科技计划项目(201702101);内蒙古卫生厅(201701097);内蒙古教育厅项目(NJZZ20168);包头市医药卫生基金(2019C3008-1);内蒙古自治区自然科学基金项目(2019BS08007)。

摘　　要：目的探讨颅缝处硬脑膜相较于普通颅骨下硬脑膜的差异表达基因,以及骨缝处硬脑膜对于细胞迁移作用的机制,为临床颅骨缝早闭合补充理论基础。方法选取6只51日龄小尾寒羊,分别取其颅缝与非颅缝下方的硬脑膜,利用二代测序检测发育期小尾寒羊的不同部位硬脑膜细胞中mRNA表达差异,进行生物信息分析,并通过RT-PCR对测序结果中mRNA表达情况进行验证。结果通过对颅缝处硬脑膜与颅骨下方硬脑膜共有的差异表达上调基因生物信息分析发现,关于骨缝处硬脑膜相对于颅骨下方硬脑膜上调的研究主要集中于细胞迁移、血管束形成以及细胞外基质产生等方面,富集程度最高的细胞通路为PI3K-AKT细胞信号通路。相关基因验证发现,PI3K-AKT细胞信号通路表达量在颅缝处下方硬脑膜较普通颅骨下方硬脑膜升高,有统计学意义(P<0.05)。结论颅骨骨缝处硬脑膜相对于颅骨下方硬脑膜PI3K-AKT细胞信号通路表达量显著升高,可能与该通路所具有的增殖与迁移功能有关。Objective To explore the differentially expressed genes of the suture dura compared with the normal subcranial dura and the mechanism of the suture dura mater on the migration of cranial suture cells,so as to provide theoretical basis for clinical suture premature closure.Methods Six 51-day-old Ovis aries were selected.The dura mater under the cranial suture and non-cranial suture were extracted,respectively.The second-generation sequencing was used to detect the differential expression of mRNA in the dural cells of different parts of the Ovis aries in the developmental stage.The biological information were analyzed.The changes of mRNA expression in the sequencing results were verified by Real-time PCR.Results Through the analysis of the biological information of the differential expression up-regulation genes shared by the dura mater at the suture and the dura below the skull,it was found that the up-regulation of the dura at the suture,relative to the dura below the skull,was mainly focused on cell migration,vascular bundle formation,and extracellular matrix production.The most enriched cell pathway was PI3K-AKT cell signaling pathway.Validation of the PI3K signaling pathway gene with high expression in the dura showed that the expression of PI3K-AKT cell signaling pathway in the dura below the suture was higher than that of the normal skull,and it was statistically significant(P<0.05).Conclusions The PI3K-AKT cell signaling pathway expression level in the dura at the suture is significantly increased compared with that below the skull,which may be related to the proliferation and migration function of the PI3K-AKT cell signaling pathway.

关 键 词：颅骨发育 颅缝 硬脑膜 mRNA 二代测序技术 

分 类 号：R651[医药卫生—外科学]