一株耐盐嗜热菌Aneurinibacillus thermoaerophilus F3的鉴定及其油脂降解特性研究  

Identification of a Halotolerant Thermophilic Bacterium Aneurinibacillus Thermoaerophilus F3 and Research on Its Characteristics of Oil Degradation

作　　者：杨玉民 范艳利 Yang Yumin;Fan Yanli(College of Food and Drug,Luoyang Normal University,Luoyang 471934,China)

机构地区：[1]洛阳师范学院食品与药品学院,河南洛阳471934

出　　处：《洛阳师范学院学报》2023年第2期13-17,共5页Journal of Luoyang Normal University

摘　　要：利用生物法高温堆肥处理高盐、高油的餐厅垃圾,符合环保理念且能够变废为宝,因此迫切需要寻求耐高盐、高油、高温又能降解油脂的嗜热菌株,以用于资源化处理餐厅垃圾.选用从土壤中筛选出的嗜热菌Aneurinibacillus thermoaerophilus F3,首先对其形态、生理生化特征进行鉴定,然后对该菌株的最适生长温度和pH值以及其耐盐能力和油脂降解特性进行研究.结果显示,F3的最适生长温度和pH值分别为50℃和7.0.该菌的最高耐盐浓度为30 g/L,在发酵培养基(油脂浓度为15 g/L)中发酵72 h,油脂降解率为58.6%,脂肪酶活性为9.12 U/mL.The use of biological high-temperature composting to deal with high-salt and high-oil restaurant waste is in line with the concept of environmental protection and can turn waste into treasure.Therefore,it is urgent to seek thermophilic strains that are resistant to high salt,high oil,high temperature and can degrade oil to deal with restaurant waste.This experiment selected the thermophilic bacteria aneurinibacillus thermoaerophilus F3 screened from soil.This research firstly identified its morphological,physiological and biochemical characteristics,and then studied its optimum growth temperature,pH value,salt tolerance,and characteristics of oil degradation.The results show that the optimum growth temperature and pH of F3 are 50°C and 7.0 respectively,and the highest salt tolerance of the strain was 30g/L.After 72-hour fermentation in the fermentation medium(oil concentration of 15g/L),the oil degradation rate is 58.6%,and the lipase activity was 9.12 U/mL.

关 键 词：油脂降解 餐厅垃圾 嗜热嗜气解硫胺素芽孢杆菌 脂肪酶 

分 类 号：X172[环境科学与工程—环境科学] X799.3