lncRNA SNHG17对肝细胞癌的增殖、侵袭和细胞周期作用的影响研究  

Effects of lncRNA SNHG17 on proliferation,invasion and cell cycle of hepatocellular carcinoma

作　　者：姚忠强[1] 王斐斐 曹霞[1] 孙琳 曲凯 牟尚东 贺礼琴 陈青娟 YAO Zhongqiang;WANG Feifei;CAO Xia;SUN Lin;QU Kai;MOU Shangdong;HE Liqin;CHEN Qingjuan(Department of Oncology,3201 Hospital Affiliated to Xi′an Jiaotong University,Hanzhong 723000;Department of Hepatobiliary Surgery,the First Affiliated Hospital of Xi′an Jiaotong University,China)

机构地区：[1]西安交通大学附属三二〇一医院肿瘤内科,陕西汉中723000 [2]西安交通大学附属第一医院肝胆外科

出　　处：《胃肠病学和肝病学杂志》2023年第3期301-306,共6页Chinese Journal of Gastroenterology and Hepatology

基　　金：陕西省自然科学基础计划研究项目(2017JM8039)。

摘　　要：目的 探讨lncRNA小核仁RNA宿主基因17(small nucleolar RNA host gene 17,SNHG17)在肝细胞癌(hepatocellular carcinoma, HCC)中的作用。方法 使用qRT-PCR检测比较90例HCC患者肿瘤组织与癌旁组织,正常人肝永生化细胞THLE-3,肝癌细胞HepG2和SNU-182系中SNHG17的表达水平差异。使用靶向SNHG17的si-SNHG17和阴性对照siRNA转染HepG2和SNU-182细胞,后分别使用CCK8分析、细胞集落形成实验、流式细胞术分析和Transwell分析不同转染细胞的功能差异。结果 与癌旁组织相比,HCC组织中SNHG17表达显著上调,与正常转化的人THLE-3细胞相比,HepG2和SNU-182细胞中SNHG17表达显著增加。SNHG17高表达组患者的肿瘤更大,TNM分期更差(P均<0.05)。与阴性对照siRNA转染相比,si-SNHG17转染的HepG2和SNU-182细胞,CCK8检测显示细胞活力明显降低,细胞集落数量显著减少,G_(0)/G_(1)期细胞比例更高,Transwell实验显示侵袭细胞数量显著减少(P均<0.05)。结论 抑制SNHG17可显著抑制肝癌细胞的活力、增殖和侵袭,导致细胞周期G_(0)/G_(1)期阻滞,可能作为未来肝癌治疗的新靶点。Objective To investigate the role of small nucleolar RNA host gene 17(SNHG17)in hepatocellular carcinoma(HCC).Methods The expression levels of SNHG17 in tumor tissues and adjacent tissues of 90 patients with HCC,normal human liver immortalized cell THLE-3,hepatoma cell HepG2 and SNU-182 were detected and compared by qRT-PCR.HepG2 and SNU-182 cells were transfected with si-SNHG17 targeting SNHG17 and negative control siRNA.The functional differences of different transfected cells were analyzed by CCK8 analysis,cell colony formation assay,flow cytometry and Transwell analysis,respectively.Results Compared with adjacent tissues,the expression of SNHG17 in HCC tissues was significantly up-regulated.Compared with normal transformed human THLE-3 cells,the expression of SNHG17 in HepG2 and SNU-182 cells was significantly increased.Patients with high expression of SNHG17 had larger tumors and worse TNM stage(P<0.05).Compared with the negative control siRNA transfection,the CCK8 detection of HepG2 and SNU-182 cells transfected with si-SNHG17 showed that the cell viability decreased significantly,the number of cell colonies decreased significantly,the proportion of G_(0)/G_(1) cells was higher,and the number of invasive cells in Transwell test decreased significantly(P<0.05).Conclusion Inhibition of SNHG17 can significantly inhibit the viability,proliferation and invasion of hepatoma cells,resulting in cell cycle G_(0)/G_(1) arrest,which may be a new target for the treatment of hepatoma in the future.

关 键 词：肝细胞癌 LncRNA SNHG17 机制 

分 类 号：R735.7[医药卫生—肿瘤]