circ_0000069靶向miR-338-3p对非小细胞肺癌A549细胞增殖、迁移和侵袭的影响  

Impacts of circ_0000069 on the proliferation,migration and invasion of non-small cell lung cancer A549 cells by targeting miR-338-3p

作　　者：张伟[1] 刘青春[1] 张潍[2] 饶汕 王辉[2] 陈奕戈 米卫国[1] ZHANG Wei;LIU Qingchun;ZHANG Wei;RAO Shan;WANG Hui;CHEN Yige;MI Weiguo(Department of Thoracic Surgery,Hanzhong Central Hospital,Hanzhong 723000,Shaanxi,China;Department of Thoracic Surgery,the Second Affiliated Hospital of Xi􀆳an Jiaotong University,Xi􀆳an 710004,Shaanxi,China)

机构地区：[1]汉中市中心医院胸外科,陕西汉中723000 [2]西安交通大学第二附属医院胸外科,陕西西安710004

出　　处：《中南医学科学杂志》2023年第2期197-201,共5页Medical Science Journal of Central South China

基　　金：陕西省自然科学基础研究计划项目(2018JQ8057)。

摘　　要：目的 探讨环状RNA 0000069(circ_0000069)是否靶向miR-338-3p调控非小细胞肺癌(NSCLC)A549细胞增殖、迁移和侵袭。方法 采用qRT-PCR技术检测NSCLC患者癌组织、癌旁正常组织中circ_0000069和miR-338-3p表达量。采用CCK-8、Transwell实验分析抑制circ_0000069或过表达miR-338-3p对A549细胞增殖活力、迁移和侵袭的影响。使用荧光素酶酶报告实验和qRT-PCR分析circ_0000069和miR-338-3p之间的相互作用。结果NSCLC组织中circ_0000069表达量较癌旁组织升高(P<0.05),而miR-338-3p表达量显著降低(P<0.05)。抑制circ_0000069或过表达miR-338-3p后A549细胞活力、迁移和侵袭细胞数显著降低(P<0.05)。miR-338-3p是circ_0000069的靶基因,circ_0000069靶向负调控miR-338-3p表达。干扰miR-338-3p表达显著减弱circ_0000069对A549细胞增殖、迁移和侵袭的抑制(P<0.05)。结论 抑制circ_0000069通过上调miR-338-3p抑制NSCLC细胞A549增殖、迁移和侵袭。Aim To investigate whether circular RNA 0000069(circ_0000069)targets miR-338-3p to regulate the proliferation,migration and invasion of non-small cell lung cancer(NSCLC)A549 cells.Methods qRT-PCR technology was used to measure the expression of circ_0000069 and miR-338-3p in cancer tissues and normal adjacent tissues of NSCLC patients.Cell counting kit method and Transwell experiment were used to analyze the impacts of inhibition of circ_0000069 or overexpression of miR-338-3p on the proliferation,migration and invasion of A549 cells.The luciferase enzyme reporter experiment and qRT-PCR were used to analyze the interaction between circ_0000069 and miR-338-3p.Results The expression of circ_0000069 in NSCLC tissue was obviously increased(P<0.05),while the expression of miR-338-3p was obviously decreased than the adjacent tissues(P<0.05).After inhibiting circ_0000069 or overexpressing miR-338-3p,the viability,migration and invasion of A549 cells were obviously reduced(P<0.05).MiR-338-3p was a target gene of circ_0000069,and circ_0000069 targeted and negatively regulated the expression of miR-338-3p.Inhibition of miR-338-3p expression obviously attenuated the impacts of inhibition of circ_0000069 on the proliferation,migration and invasion of A549 cells(P<0.05).Conclusion Inhibition of circ_0000069 can inhibit the proliferation,migration and invasion of NSCLC cells A549 by up-regulating miR-338-3p.

关 键 词：circ_0000069 miR-338-3p 非小细胞肺癌 细胞增殖 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]