三种宽瓣亚属植物生境土壤真菌群落特征  

Characteristics of soil fungi community in wild populations of three Paphiopedilum Subgen Brachypetalum species

作　　者：田力 安明态[1] 张央 吴墨栩 TIAN Li;AN Ming-tai;ZHANG Yang;WU Mo-xu(Center for Biodiversity and Natural Conservation,College of Forestry,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学林学院,贵州大学生物多样性与自然保护研究中心,贵州贵阳550025

出　　处：《中国环境科学》2023年第4期1995-2006,共12页China Environmental Science

基　　金：国家自然科学基金资助项目(31960042)。

摘　　要：以杏黄兜兰、文山兜兰、同色兜兰为研究对象,调查生境信息,采用ITS高通量测序对生境土壤真菌群落结构、真菌功能群注释及多样性进行分析,并揭示环境因子的影响.结果显示,3种兜兰生境土壤样品共获得1105个可操纵分类单元(OTU),包含322种真菌,隶属10门29纲70目151科256属,3种兜兰生境土壤真菌群落中出现48个共有OTU,同色兜兰含489个特有OTU,文山兜兰有221个,杏黄兜兰202个,3种兜兰生境土壤真菌群落在门、纲、科、属水平均存在不同优势类群,FUNGuild真菌功能群注释可知,3种兜兰生境土壤真菌功能群均以腐生真菌、外生菌根为主,同色兜兰生境土壤真菌功能类群更加复杂;3种兜兰之间土壤真菌的ACE指数与Chao1指数无明显差异,而同色兜兰Simpson指数与Shannon指数显著高于杏黄兜兰,且极显著高于文山兜兰.Beta多样性发现3种兜兰生境土壤真菌群落组成与结构差异明显(P=0.001),且分类水平越高,差异越大;冗余分析揭示了经纬度与植被类型是对3种兜兰生境土壤群落变化影响最大的因子,所选环境因子共揭示真菌群落变化的44.08%.mental检验显示土壤pH值显著影响3种兜兰生境土壤真菌的alpha多样性.Spearman相关性分析证实多种真菌优势类群与土壤理化因子的显著相关.以上研究表明,杏黄兜兰、文山兜兰、同色兜兰的土壤真菌群落存在不同的结构特征、真菌功能群,真菌多样性存在显著差异.环境因子导致了3种兜兰生境土壤真菌群落结构变化,而土壤理化性质影响了真菌群落优势类群的分布.Paphiopedilum armeniacum,Paphiopedilum wenshanense and Paphiopedilum concolor as the research object,research habitat information,uncover the structural characteristics of soil fungal compositions and the associated environmental factors would provide the basis for efficient protection and cultivation.In this study,we investigated the habitat parameters and soil fungal community using samples collected from the natural habitats of the three species.The structural characteristics,diversity of fungal communities,fungal functional groups and the associated environmental factors were preliminarily analyzed by ITS high-throughput sequencing.A total of 1105 operational taxonomic units(OTUs)were obtained from 27 samples,322 fungi were identified,belonging to 10 phyla,29 classes,70 orders,151 families,256 genera,respectively.There were 48 common OTUs in the habitat of three Subgen Brachypetalum species,489 unique OTUs in P.concolor,221 in P.wenshanense and 202 in P.armeniacum.There were different dominant groups at the taxonomic level of phylum,class,family and genus in the habitat of three Subgen Brachypetalum species.The fungal functional groups annotation found that the soil fungal functional groups in the habitat of three Subgen Brachypetalum species were dominated by saprophytic fungi and exogenous mycorrhizal fungi.The functional groups of habitats of P.concolor are more complex than other two species;Alpha diversity analysis results showed the congruence in the ACE index and Chao1index.The Simpson index and Shannon index of P.concolor were significantly higher than those of P.armeniacum,and were extremely significantly higher than those of P.wenshanense.Beta diversity analysis showed that there were significant differences in soil fungal community composition and structure among the habitat of three Subgen Brachypetalum species(P=0.001),and the higher the classification level.Redundancy analysis found that latitude and longitude and vegetation types were the most important factors affecting the changes of soil com

关 键 词：宽瓣亚属 高通量测序 土壤真菌群落 生境 

分 类 号：X172[环境科学与工程—环境科学]