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作 者:王招娣 赵佳佳 杨荷雨 谢敏 刘玲[1] WANG Zhaodi;ZHAO Jiajia;YANG Heyu;XIE Min;LIU Ling(School of Pharmacy,Hubei University of Science and Technology,Xianning 437100,Hubei,China)
机构地区:[1]湖北科技学院医学部药学院,湖北咸宁437100
出 处:《西部医学》2023年第4期490-494,共5页Medical Journal of West China
基 金:国家自然科学基金项目(32100823)。
摘 要:目的 探究EphB1-Fc靶向阻断EphB受体介导的信号在抑制慢性炎症痛中的作用,并探讨其病理机制。方法 18只SPF级雄性SD大鼠随机分为对照组(Control组)、CFA模型组(CFA组)及CFA和EphB1-Fc联合处理组(CFA+EphB1-Fc组),每组6只。在模型组和联合处理组大鼠左侧足底皮下注射50μL完全弗氏佐剂(CFA),使注射区域产生红、肿、热和痛等炎症反应。在对照组大鼠同侧足底皮下注射等剂量的生理盐水。模型构建7 d后,联合处理组鞘内注射5μg EphB1-Fc(10μL),其余两组大鼠鞘内注射等量的生理盐水。记录3组大鼠痛觉行为变化;免疫荧光和免疫印迹分析3组大鼠脊髓组织中EphB信号变化及脊髓炎症相关蛋白水平变化。结果 EphB1-Fc可显著缓解CFA诱导的大鼠机械痛敏,降低脊髓组织中炎症相关因子IL-1β表达;EphB1-Fc给药可阻断EphB1/Src/NF-κB信号。结论 靶向阻断EphB1介导的Src/NF-κB信号降低脊髓炎症缓解病理性疼痛。Objective To investigate the inhibitory effect and mechanism of EphB1-Fc targeted blocking EphB receptor-mediated signal on chronic inflammatory pain.Methods A rat model of inflammatory pain was established by intraplantar injection with 50μl CFA in the left hind paw.The rats were randomly divided into Control group,CFA group and CFA+EphB1-Fc group.Spontaneous pain(flinches)and mechanical pain threshold(PWT)were used to detect the changes of pain behavior of rats.Immunofluorescence and Western blotting were used to detect the changes in the level of EphB signal and inflammatory factors in spinal cord.Results Intrathecally injection of EphB1-Fc significantly alleviated CFA induced hyperalgesia in rats.EphB1-Fc injection obviously inhibited the activation spinal inflammatory factor IL-1β.EphB1-Fc blocked EphB1/SRC/NF-κB signal pathway.Conclusion Blocking EphB1 mediates SRC/NF-κB signal decreases spinal inflammation and alleviates pathological pain.
分 类 号:R741.02[医药卫生—神经病学与精神病学]
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