甲状腺癌组织中miR-93-5p、BRMS1L的表达水平及其临床意义  

Expression of mir-93-5p and BRMS1L in Thyroid Carcinoma and Its Clinical Significance

作　　者：王思寅 董颖[1] 滕立勤 WANG Siyin;DONG Ying;TENG Liqin(The Second Affiliated Hospital of Zhengzhou University,Zhengzhou,450000)

机构地区：[1]郑州大学第二附属医院,450000

出　　处：《实用癌症杂志》2023年第4期538-541,共4页The Practical Journal of Cancer

摘　　要：目的 探讨微小RNA-93-5p(miR-93-5p)与乳腺癌转移抑制基因1相似基因(BRMS1L)在甲状腺癌组织内的表达与临床意义。方法 选取68例甲状腺癌患者,术中采集甲状腺癌组织与癌旁正常组织,以逆转录-聚合酶链反应法(RT-PCR)与免疫组织化学法分别测定miR-93-5p与BRMS1L表达水平;分析miR-93-5p、BRMS1L表达与患者性别、年龄、体重指数(BMI)、TNM分期、淋巴结转移、分化程度等病理特征之间的关系。另外,随访3年,以Kaplan-Meier法分析miR-93-5p、BRMS1L表达与患者预后的关系。结果 癌组织miR-93-5p相对表达量高于癌旁组织,BRMS1L阳性表达率低于癌旁组织,有统计学差异(P<0.05)。miR-93-5p、BRMS1L表达与患者的性别、BMI无关(P>0.05),与患者的年龄、TNM分期、淋巴结转移、分化程度有关(P<0.05)。Kaplan-Meier结果显示:随访3年内,miR-93-5p高表达患者[47.62%(20/42)]与BRMS1L阴性表达患者[51.11%(23/45)]的3年生存率低于miR-93-5p低表达患者[73.08%(19/26)]与BRMS1L阳性表达患者[78.26%(18/23)]。结论 甲状腺癌组织内miR-93-5p、BRMS1L呈异常表达,两者表达与年龄、TNM分期、淋巴结转移、分化程度密切相关,且miR-93-5p高表达与BRMS1L低表达患者生存率更低,预后更差。Objective To investigate the expression and clinical significance of microrna-93-5p(mir-93-5p) and breast cancer metastasis suppressor gene 1(BRMS1L) in thyroid cancer.Methods 68 patients with thyroid cancer diagnosed and treated were selected.The expression levels of mir-93-5p and BRMS1L were measured by reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry;To analyze the relationship between the expression of mir-93-5p and BRMS1L and the pathological characteristics of patients such as gender,age,body mass index(BMI),TNM stage,lymph node metastasis and differentiation degree;In addition,the relationship between the expression of mir-93-5p and BRMS1L and the prognosis of patients was analyzed by Kaplan Meier method.Results the relative expression of mir-93-5p in cancer tissues was higher than that in adjacent tissues,and the positive expression rate of BRMS1L was lower than that in adjacent tissues(P<0.05);The expression of mir-93-5p and BRMS1L had no correlation with the gender and BMI of the patients(P>0.05);The expression of mir-93-5p and BRMS1L was correlated with the age,TNM stage,lymph node metastasis and differentiation of the patients(P<0.05).Kaplan Meier results showed that the 3-year survival rate of patients with high expression of mir-93-5p [47.62%(20/42)] and patients with negative expression of BRMS1L [51.11%(23/45)] was lower than that of patients with low expression of mir-93-5p[73.08%(19/26)] and patients with positive expression of BRMS1L [78.26%(18/23)].Conclusion The expression of mir-93-5p and BRMS1L is abnormal in thyroid carcinoma.The expression of mir-93-5p and BRMS1L is closely related to age,TNM stage,lymph node metastasis and differentiation.The patients with high expression of mir-93-5p and low expression of BRMS1L have lower survival rate and worse prognosis.

关 键 词：甲状腺癌 微小RNA-93-5p 乳腺癌转移抑制基因1相似基因 临床病理特征 预后 

分 类 号：R736.1[医药卫生—肿瘤]