基于网络药理学和药理实验的活血祛湿方治疗脂肪肝作用机制研究  

Mechanism of Huoxue Qushi Fomula in Treating Fatty Liver Based on Network Pharmacology and Pharmacological Experiments

作　　者：班亭玉 卢秉久[2] BAN Tingyu;LU Bingjiu(Graduate School of Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;Department of Infectious Diseases of the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110033,China)

机构地区：[1]辽宁中医药大学研究生学院,沈阳110847 [2]辽宁中医药大学附属医院感染科,沈阳110033

出　　处：《世界中医药》2023年第4期451-457,共7页World Chinese Medicine

基　　金：全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号);辽宁省教育厅科学研究技术项目(L201903)。

摘　　要：目的:分析活血祛湿方的有效活性成分及其治疗脂肪肝的分子机制。方法:分别通过中药系统药理学数据库与分析平台(TCMSP)、GeneCards数据库查找活血祛湿方中10味中药的有效活性成分及其靶点蛋白和人类脂肪肝相关的作用靶点,构建有效活性成分-疾病靶点的网络图;通过String数据库进行构建蛋白质-蛋白质相互作用(PPI)网络,通过Metascape数据库进行基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)富集分析;最后使用分子对接技术和药理实验对有效活性成分和靶点进行验证。结果:经过筛选,活血祛湿方中的槲皮素、山柰酚、木犀草素、柚皮素以及异鼠李素等多个关键活性成分,可能通过作用在信号转导与转录激活因子3、RAC-α丝氨酸/苏氨酸蛋白激酶、细胞肿瘤抗原p53、转录因子AP-1等核心基因表达的蛋白上参与脂肪肝的治疗。KEGG富集分析所涉及的信号通路包括白细胞介素-17(IL-17)、前列腺癌、缺氧诱导因子-1(HIF-1)、非酒精性脂肪肝、促分裂原活化的蛋白激酶(MAPK)信号通路等。结论:活血祛湿方可通过多成分、多靶点、多通路的复杂作用机制发挥抗炎和应对氧化应激的作用,进而对脂肪肝起到治疗作用。Objective:To analyze the active components of Huoxue Qushi Fomula in treating fatty liver and explore its mechanism.Methods:Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and Genecards were searched to screen the active components of Huoxue Qushi Fomula and their target proteins and the targets related to human fatty liver.Then,the network of active components and disease targets was constructed,and String was used to construct protein-protein interaction(PPI) network.GO enrichment analysis and KEGG enrichment analysis were carried out by Metascape.Finally,the active components and targets were verified by molecular docking and pharmacological experiments.Results:After screening,multiple key active components of Huoxue Qushi Fomula(including quercetin,kaempferol,luteolin,naringenin,and isorhamnetin) might be effective for the treatment of fatty liver through signal transducer and activator of transcription 3(STAT3),serine/threonine kinase 1(AKT1),tumor protein p53(TP53),transcription factor AP-1(JUN),etc.The pathways involved in KEGG enrichment analysis included interleukin-17(IL-17),prostate cancer,hypoxia-inducible factor-1(HIF-1),non-alcoholic fatty liver,and mitogen-activated protein kinase(MAPK) signaling pathways.Conclusion:Huoxue Qushi Fomula resists inflammation and oxidative stress via complex multi-component,multi-target,and multi-pathway mechanism,thus treating fatty liver.

关 键 词：活血祛湿方 脂肪肝 网络药理 分子对接 作用机制 药理实验 

分 类 号：R285.5[医药卫生—中药学]