固相萃取-高效液相色谱法测定奶粉中多环芳烃  

Determination of polycyclic aromatic hydrocarbons in milk powder by solid phase extraction-high performance liquid chromatography

作　　者：郭新颖[1] 陆娟[1] GUO Xinying;LU Juan(Nantong Center for Disease Control and Prevention,Nantong 226001,China)

机构地区：[1]南通市疾病预防控制中心,江苏南通226001

出　　处：《化学分析计量》2023年第3期18-21,共4页Chemical Analysis And Meterage

基　　金：江苏省中医药局项目(YB2020068);南通市科技计划基础科学研究项目(JC2021076);南通市卫健委青年基金项目(QA2021054)。

摘　　要：建立固相萃取-高效液相色谱法测定配方奶粉中多环芳烃的方法。样品经水提取、乙腈粗去蛋白,提取液经优化配比的MWCNTs和PSA固相萃取净化,用高效液相色谱荧光检测器测定。结果表明,11种多环芳烃的方法检出限为0.10~0.75μg/kg,低、中、高三个浓度水平的加标回收率范围为86.3%~97.4%,测定结果的相对标准偏差为2.4%~7.3%(n=6)。该方法简单快速、准确,适用于对奶粉样品中11种多环芳烃测定要求,可为食品卫生监管部门提供参考方法。A solid phase extraction-high performance liquid chromatography(HPLC)was established for the determination of polycyclic aromatic hydrocarbons(PAHs)in formula milk powder.The sample was extracted with water and crude protein was removed with acetonitrile.The extract was purified by solid phase extraction with MWCNTs and PSA in the optimized ratio,and analyzed by HPLC with fluorescence detector.The detection limit of 11 PAHs was 0.10-0.75μg/kg,the recovery range of low,medium and high concentration levels addition was 86.3%-97.4%,and the relative standard deviation of determination results was 2.4%-7.3%(n=6).The method is simple,rapid,accurate,which is suitable for the determination of 11 PAHs in the actual samples of milk powder,and it provides a reference method for the food hygiene supervision department.

关 键 词：多环芳烃 奶粉 高效液相色谱法 荧光检测器 

分 类 号：O657.7[理学—分析化学]