玉米蛋白水解物的酶法制备及其对结肠炎的缓解作用  

Enzymatic Preparation of Corn Protein Hydrolysate and Its Alleviating Effect on Colitis

作　　者：景言 刘晓兰 王今雨 赵玉昊 马永强[1] 郑喜群 JING Yan;LIU Xiaolan;WANG Jinyu;ZHAO Yuhao;MA Yongqiang;ZHENG Xiqun(College of Food Engineering,Harbin University of Commerce,Harbin 150028,China;Key Laboratory of Corn Deep Processing Theory and Technology of Heilongjiang Province,College of Food and Bioengineering,Qiqihar University,Qiqihar 161006,China;College of Food,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028 [2]齐齐哈尔大学食品与生物工程学院,黑龙江省玉米深加工理论与技术重点实验室,黑龙江齐齐哈尔161006 [3]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《食品工业科技》2023年第8期270-278,共9页Science and Technology of Food Industry

基　　金：黑龙江省重点研发计划指导类项目(GZ20210097);中央支持地方高校改革发展资金高水平人才项目(2020GSP08);十四五国家重点研发计划项目子课题(2021YFD2100900);黑龙江省省属本科高校基本科研业务费项目(135509223)。

摘　　要：为了探究玉米蛋白水解物对肠道屏障功能的保护作用,本文首先以玉米蛋白粉为原料,采用Alcalase对其进行酶解,考察了不同酶解条件对水解物中谷氨酰胺含量的影响,建立了玉米蛋白的最适水解体系。然后以葡聚糖硫酸钠为诱导剂构建小鼠结肠炎模型,系统的评价了玉米蛋白水解物对小鼠结肠炎的缓解作用。结果表明:Alcalase的最适酶解条件为酶解温度64℃,酶解时间2.5 h,加酶量1300 U/g;在此条件下,水解物中谷氨酰胺含量可达到8.79%。所制得的玉米蛋白水解物(375和625 mg/kg·bw)能够明显改善结肠炎小鼠体重下降,缓解结肠缩短,降低疾病活动指数,减轻结肠组织中炎症细胞的浸润,调节炎症细胞因子水平。玉米蛋白水解物对小鼠结肠炎具有良好的缓解作用,本研究旨为拓宽玉米蛋白粉的高值化利用及开发肠道保护的功能性食品提供理论依据。In order to explore the protective effect of corn protein hydrolysate on intestinal barrier function,corn gluten meal was used as raw material and Alcalase was used as catalyst for enzymatic hydrolysis in current study.The effects of different enzymatic hydrolysis conditions on the content of glutamine in the hydrolysate were investigated,and the optimum hydrolysis system of corn protein was established.Then the sodium dextran sulfate was used as inducer to construct colitis model,and the alleviating effect of corn protein hydrolysate on colitis in mice was systematically evaluated.The results showed that the optimum hydrolysis conditions for Alcalase were a temperature of 64℃,hydrolysis time of 2.5 h,amount of enzyme of 1300 U/g.Under such condition,the content of glutamine in the hydrolysate could reach 8.79%.Corn protein hydrolysate(375 and 625 mg/kg·bw)significantly improved weight loss in colitis mice,reduced the colonic shortening and the disease activity index,diminished the infiltration of inflammatory cells in the colonic tissue,regulated the level of inflammatory cytokines,and played a good role in relieving colitis in mice.This study would provide theoretical basis for expanding high-value utilization of corn gluten meal and developing functional food for intestinal protection.

关 键 词：玉米蛋白粉 玉米蛋白水解物 葡聚糖硫酸钠 结肠炎 

分 类 号：TS239[轻工技术与工程—粮食、油脂及植物蛋白工程]