MT1-MMP基因单核苷酸多态性与广西壮族绝经后女性骨质疏松的相关性  

Association between MTI-MMP single nucleotide polymorphisms and osteoporosis in postmenopausal women of Zhuang Nationality in Guangxi

作　　者：周庆辉 黄秀峰 黄昌盛 马福维 杨园园 王金花[2] ZHOU Qing-Hui;HUANG Xiu-Feng;HUANG Chang-Sheng(Department of Anatomy,School of Basic Medicine,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院基础医学院人体解剖学教研室,广西百色533000 [2]右江民族医学院组织胚胎学教研室,广西百色533000

出　　处：《中国老年学杂志》2023年第8期1847-1851,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81260071,81560239);广西自然科学基金资助项目(2013GXNSFAA019220)。

摘　　要：目的 研究膜型基质金属蛋白酶-1(MT1-MMP)基因单核苷酸多态性与广西壮族绝经后女性骨质疏松的关系。方法 用超声骨密度仪测量广西地区518例壮族绝经后女性左侧跟骨超声振幅衰减(BUA),并根据中国人骨质疏松建议诊断标准分骨量正常、骨量减少和骨质疏松组;MT1-MMP基因的4个单核苷酸多态性(SNP)位点(rs1003349、rs3751488、rs2236303和rs743257)的分型采用多重单碱基延伸SNP分型技术(Multiplex SNaPshot)。结果 4个位点单核苷酸多态性与BUA变异无相关性(P>0.05)。rs743257位点TT基因型在骨量减少组和骨质疏松组中的分布频率明显比骨量正常组低,CT基因型则相反;在对骨质疏松危险因素的有序Logistic回归分析显示,壮族绝经后女性个体携带rs2236303 CT基因型发生骨质疏松的风险比TT基因型的低(OR=0.532,95%CI:0.308~0.917,P=0.023),携带rs743257 CT基因型发生骨质疏松的风险比TT基因型的高(OR=1.873,95%CI:1.159~3.027,P=0.010)。结论 MT1-MMP基因rs2236303 C/T和rs743257 C/T的基因多态性可能与广西壮族绝经后女性骨质疏松相关,rs2236303 TT基因型和rs743257 CT基因型可能是增加骨质疏松风险的因素。Objective To study the relationship between single nucleotide polymorphism of membrane matrix metalloproteinase-1(MT1-MMP)gene and osteoporosis in postmenopausal women of Zhuang Nationality in Guangxi.Methods 518 postmenopausal women of Zhuang Nationality in Guangxi were enrolled to measure the left calcaneal broadband ultrasonic attenuation(BUA)with ultrasound bone densitometer.According to the recommended diagnostic criteria of osteoporosis in Chinese,they were divided into three groups:normal control group,osteopenia group and osteoporosis group.Four single nucleotide polymorphism(SNP)loci(rs1003349,rs3751488,rs2236303 and rs743257)of MTI-MMP gene were performed using Multiplex SNaPshot technology.Results There was no correlation between four single nucleotide polymorphisms and BUA variation(P>0.05).The distribution frequency of rs743257 TT genotype in osteopenia group and osteoporosis group was significantly lower than that in normal group,while that of CT genotype was on the contrary.The ordered Logistic regression analysis of risk factors of osteoporosis showed that the risk of osteoporosis in postmenopausal women of Zhuang Nationality with rs2236303 CT genotype was lower than that with TT genotype(OR=0.532,95%CI:0.308~0.917,P=0.023),the risk of osteoporosis with rs743257 CT genotype was higher than that with TT genotype(OR=1.873,95%CI:1.159~3.027,P=0.010).Conclusions The rs2236303 C/T and rs743257 C/T polymorphisms of MTI-MMP gene might be associated with osteoporosis in postmenopausal women of Zhuang Nationality in Guangxi,rs2236303 TT genotype and rs743257 CT genotype might be the factors that increase the risk of osteoporosis.

关 键 词：膜型基质金属蛋白酶-1 单核苷酸多态性 骨质疏松 绝经后女性 壮族 

分 类 号：R394.5[医药卫生—医学遗传学]