肺癌根治术后感染对机体JAK/STAT通路蛋白及T细胞亚群的影响  

Effects of infection on JAK/STAT pathway proteins and T cell subsets in vivo after radical resection of lung cancer

作　　者：魏一泽 王宾宾 黄文军[1] 高从荣[1] 董明杰 WEI Yize;WANG Binbin;HUANG Wenjun;GAO Congrong;DONG Mingjie(Department of Cardiothoracic Surgery,Hefei Second People’s Hospital,Hefei,Anhui,China,230011)

机构地区：[1]合肥市第二人民医院胸心外科,安徽合肥230011

出　　处：《分子诊断与治疗杂志》2023年第3期493-496,500,共5页Journal of Molecular Diagnostics and Therapy

基　　金：合肥市第二人民医院院级科研项目(2021yqn10)。

摘　　要：目的探讨肺癌根治术后感染对机体JAK/STAT通路蛋白及T细胞亚群的影响。方法选取2020年1月至2021年12月于合肥市第二人民医院胸外科收治的行肺癌根治术的114例患者作为研究对象。按患者术后是否发生肺部感染分为发生组(n=24)和未发生组(n=90),统计分析两组肺组织中JAK/STAT相关通路蛋白[EFhD2、磷酸化JAK2(p-JAK2)、JAK2、p-STAT3、STAT3]表达量、外周血T淋巴细胞亚群[CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)]、炎症反应因子[胰岛素样生长因子-1(IGF-1)、和肽素、Ⅳ型胶原(Ⅳ-C)]及早期预后情况。结果与未发生组相比,发生组肺组织中EFhD2、磷酸化JAK2(p-JAK2)、JAK2、p-STAT3、STAT3的表达均上调,差异有统计学意义(t=5.005、2.261、2.657、4.189、3.625,P<0.05)。与未发生组相比,发生组外周血T细胞亚群CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)水平均降低,差异有统计学意义(t=2.230、2.344、2.162、5.377,P<0.05)。与未发生组相比,发生组炎症反应因子IGF-1、和肽素、Ⅳ-C水平均升高,差异有统计学意义(t=1.999、2.269、5.663,P<0.05)。与未发生组相比,发生组恶心呕吐发生率更高,差异有统计学意义(χ^(2)=5.296,P<0.05)。结论肺癌根治术后感染能够明显影响患者体JAK/STAT通路蛋白、T细胞亚群及相关炎症指标,可能影响肺癌患者预后。可能影响患者肺癌的远期预后?还是此次感染后的预后。Objective To investigate the effect of infection after radical resection of lung cancer on JAK/STAT pathway protein and T cell subsets.Methods A total of 114 patients who underwent radical resection of lung cancer in the Department of Thoracic Surgery of Hefei Second People's Hospital from January 2020 to December 2021 were selected as the study subjects.The patients were divided into the occurrence group(n=24)and the non⁃occurrence group(n=90)according to whether the postoperative pulmonary infection occurred or not.The expression of JAK/STAT related pathway proteins[EFhD2,phosphorylated JAK2(p⁃JAK2),JAK2,p⁃STAT3,STAT3],peripheral blood T lymphocyte subsets[CD3^(+),CD4^(+),CD8^(+),CD4^(+)/CD8^(+)],inflammatory response factors[insulin⁃like growth factor⁃1(IGF⁃1),copeptin,typeⅣcollagen(Ⅳ⁃C)]and early prognosis were statistically analyzed.Results Compared with the non⁃occurrence group,the expression of EFhD2,phosphorylated JAK2(p⁃JAK2),JAK2,p⁃STAT3 and STAT3 in the lung tissue of the occurrence group was up⁃regulated,and the difference was statistically significant(t=5.005,2.261,2.657,4.189,3.625,P<0.05).Compared with the non⁃occurrence group,the levels of CD3^(+),CD4^(+),CD8^(+)and CD4^(+)/CD8^(+)in peripheral blood T cell subsets in the occurrence group decreased,and the difference was statistically significant(t=2.230,2.344,2.162,5.377,P<0.05).Compared with the non⁃occurrence group,the levels of inflammatory response factors IGF⁃1,copeptin andⅣ⁃C in the occurrence group were increased,and the differences were statistically significant(t=1.999,2.269,5.663,P<0.05).Compared with the non⁃occurrence group,the incidence of nausea and vomiting in the occurrence group was higher,the difference was statistically significant(χ^(2)=5.296,P<0.05).Conclusion Infection after radical resection of lung cancer can significantly affect JAK/STAT pathway protein,T cell subsets and related inflammatory indicators,which may affect the prognosis of patients with lung cancer.

关 键 词：肺癌 术后肺部感染 JAK/STAT通路蛋白 T细胞亚群 

分 类 号：R734.2[医药卫生—肿瘤]