合生元对CCl_(4)诱发小鼠急性化学性肝损伤的改善作用及其机制  

Liver protective effect and mechanism of synbiotics on mice with CCl4-induced acute chemical liver injury

作　　者：李艳艳 丁红[1] 刘承尧 郭宇沨 LI Yanyan;DING Hong;LIU Chengyao;GUO Yufeng(School of Public Health,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐830011

出　　处：《山东医药》2023年第11期37-42,共6页Shandong Medical Journal

基　　金：新疆医科大学研究生科研创新项目(CXCY2022036)。

摘　　要：目的探讨不同组合的合生元对CCl_(4)所致急性化学性肝损伤小鼠的肝脏保护作用,及其机制是否与肠—肝轴有关。方法将72只SPF级ICR雄性小鼠随机分12组,每组6只。将含量为5%、15%和20%的麦麸膳食纤维饲料分别记为A1、A2、A3,灌胃剂量为10、20、30 mL/kg的益生菌酸奶分别记为B1、B2、B3,对A1B1组、A1B2组、A1B3组、A2B1组、A2B2组、A2B3组、A3B1组、A3B2组、A3B3组分别进行不同组成合生元干预,阳性对照组、模型组和空白对照组给予普通饲料,阳性对照组同时灌胃复方鳖甲软肝片溶液;30 d后,除空白对照组外的其余各组小鼠灌胃给予1%的CCl_(4)油溶液5 mL/kg,空白对照组灌胃给予等体积玉米油溶液。24 h后,全部小鼠摘眼球取血并分离血清,采用ELISA法检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平;取肝脏、结肠、回肠,计算肝脏指数和结肠指数,同时对肝脏和回肠进行HE染色后观察病理改变;收集A1B1组、阳性对照组、模型组和空白对照组小鼠粪便,采用16S rDNA高通量测序法检测肠道菌群情况(Chao、Ace、Shannon、Simpson指数及门水平、属水平上的肠道菌群丰度)。结果模型组肝脏指数及血清MDA水平均高于空白对照组(P均<0.05)。A1B1、A1B2、A2B1和A3B1组小鼠肝脏指数均低于模型组,A1B1、A2B1组小鼠肝脏指数均低于阳性对照组(P均<0.05)。各组小鼠结肠指数比较差异无统计学意义(P>0.05)。A2B1、A2B3组小鼠血清SOD水平均低于模型组、阳性对照组,A1B1、A1B2、A2B3、A3B1和A3B2组小鼠血清MDA水平均低于模型组(P均<0.05);除A1B2组外,其余合生元组小鼠血清MDA水平均高于阳性对照组(P均<0.05)。肝脏病理观察结果:空白对照组小鼠肝组织细胞排列紧密且形态正常,细胞结构清晰且完整,未见炎症细胞浸润和坏死细胞;模型组小鼠肝索、肝小叶结构严重紊乱,细胞肿胀、细胞内存在大量未代谢的CCl_(4);阳性对照组肝细胞�Objective To explore the liver protective effect of different combinations of synbiotics on mice with CCl_(4)-induced acute chemical liver injury,and to explore whether the mechanism is related to the gut-liver axis.Methods Seventy-two SPF grade ICR male mice were randomly divided into 12 groups,with six in each.The wheat bran dietary fiber of 5%,15%,and 20%were recorded as A1,A2 and A3,respectively,probiotic yogurt with gastric infusion doses of 10,20,and 30 mL/kg were recorded as B1,B2 and B3,respectively.Mice in the A1B1,A1B2,A1B3,A2B1,A2B2,A2B3,A3B1,A3B2 and A3B3 groups underwent synbiotics intervention of different combinations.Mice in the positive control group,model group and blank control group were given common feed,and mice in the positive control group were simultaneously treated with compound turtle shell soft liver tablet solution.After 30 d,mice of each group except the blank control group were given 5 mL/kg of 1%CCl_(4) oil solution,and mice in the blank control group were given equal volume of corn oil solution.After 24 h,the eyeballs of all mice were removed to collect blood and the serum was isolated.Serum su-peroxide dismutase(SOD)and malondialdehyde(MDA)levels were measured by ELISA.The liver,colon and ileum were taken to calculate the liver index and colon index,and the pathological changes were observed after HE staining of the liver and ileum;the feces of A1B1 group,positive control group,model group and blank control group were collected,and their intestinal flora were analyzed by 16S rDNA high-throughput sequencing(Chao,Ace,Shannon,Simpson indices and abundance of gut microbiota at the phylum and genus levels).Results The liver index and serum MDA level were high-er in the model group than in the blank control group(both P<0.05).The liver indices of mice in the A1B1,A1B2,A2B1 and A3B1 groups were lower than those of the model group,and the liver indices of mice in the A1B1 and A2B1 groups were lower than those of the positive control group(all P<0.05).There was no significant difference i

关 键 词：合生元 CCl_(4) 肠道菌群 肝功能 肠—肝轴 急性化学性肝损伤 

分 类 号：R151.3[医药卫生—营养与食品卫生学]