基于TLR4/NF-κB/IL-1β通路研究人参皂苷Rg3对缓解慢性炎症痛的作用机制  

Mechanism of ginsenoside Rg3 in relieving chronic inflammatory pain based on TLR4/NF-κB/IL-1βpathway

作　　者：孙旭 朱海丽 谢敏 高卉[1] SUN Xu;ZHU Haili;XIE Min;GAO Hui(School of Pharmacy,Hubei University of Science and Technology,Xianning,Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100

出　　处：《现代医药卫生》2023年第7期1085-1088,1093,共5页Journal of Modern Medicine & Health

基　　金：国家自然科学基金项目(81901149)。

摘　　要：目的研究人参皂苷Rg3缓解慢性炎症痛的作用机制。方法采用脚掌注射完全弗氏佐剂构建慢性炎症痛小鼠模型,鞘内注射人参皂苷Rg3进行脊髓给药。采用完全随机方法分为对照组、模型组和人参皂苷Rg3组,每组10只。连续给药3 d后观察小鼠足肿胀、痛觉行为及体能变化;采用免疫印迹法检测各组小鼠脊髓组织蛋白表达量。结果给予完全弗氏佐剂干预后,小鼠各项指标均有不同程度变化,脊髓组织星形胶质细胞标志物——神经胶质纤维酸性蛋白、白细胞介素1β(IL-1β)、细胞Toll样受体4(TLR4)、髓样分化因子88和核因子κB(NF-κB)蛋白表达水平均增加。人参皂苷Rg3鞘内给药后,减少了小鼠自发缩足次数,提高了机械痛阈值,延长了转棒停留时间及运动距离,使神经胶质纤维酸性蛋白、IL-1β、TLR4、髓样分化因子88、NF-κB蛋白表达水平被抑制。结论人参皂苷Rg3通过降低TLR4/NF-κB/IL-1β炎症信号缓解慢性炎症痛。Objective To study the effect and mechanism of ginsenoside Rg3 on chronic inflammatory pain.Methods The mouse models of chronic inflammatory pain were established by injecting complete Freund′s adjuvant into the sole of the foot,and ginsenoside Rg3 was injected intrathecally for spinal cord administration.The mice were randomly divided into the control group,the model group and the ginsenoside Rg3 group,10 mice in each group.After three days of continuous administration,the changes of foot swelling,pain behavior and physical ability of the mice were observed.The expression of protein in spinal cord tissue of the mice in each group was detected by western blotting.Results After complete Freund′s adjuvant intervention,all indexes of the mice changed in different degrees.The expression levels of astrocyte markers in spinal cord tissue:glial fibrillary acidic protein(GFAP),interleukin-1β(IL-1β),toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88)and nuclear factor kappa B(NF-κB)all increased.Intrathecal administration of ginsenoside Rg3 reduced the number of spontaneous foot contraction in mice,increased the threshold of mechanical pain,increased the stay time and movement distance of rotating rod,and inhibited the expression levels of GFAP,IL-1β,TLR4,MyD88 and NF-κB protein.Conclusion Ginsenoside Rg3 can relieve chronic inflammatory pain by reducing the inflammatory signal of TLR4/NF-κB/IL-1β.

关 键 词：人参皂苷RG3 慢性炎症痛 细胞Toll样受体4 核因子ΚB 白细胞介素1Β 

分 类 号：R741.02[医药卫生—神经病学与精神病学] R441.1[医药卫生—临床医学]