姜黄素类似物对人结肠癌HT-29细胞增殖与自噬的影响  

Effects of curcumin analogue on proliferation and autophagy of human colon cancer HT-29 cells

作　　者：黄屹沅 陈云[1] 陈瑞骥 王威[1] HUANG Yiyuan;CHEN Yun;CHEN Ruiji;WANG Wei(Department of Pharmacy,Taizhou Hospital of Traditional Chinese Medicine,Taizhou,Jiangsu 225300,China;Hainan Hospital Affiliated to General Hospital of PLA,Sanya,Hainan 572022,China)

机构地区：[1]泰州市中医院药学部,江苏泰州225300 [2]解放军总医院海南医院,海南三亚572022

出　　处：《现代医药卫生》2023年第7期1094-1099,共6页Journal of Modern Medicine & Health

基　　金：国家中医临床研究基地;江苏省中医药管理局科技项目资助;江苏省中医药研究院开放课题基金项目(JD2019SZXYB21)。

摘　　要：目的初步探究姜黄素类似物——WH-8对人结肠癌HT-29细胞增殖和自噬的影响。方法选用不同浓度WH-8作用于人结肠癌HT-29细胞,应用四甲基偶氮唑盐还原法观察药物对细胞的生长抑制作用;通过荧光染色技术和细胞成像技术观察WH-8作用下细胞自噬囊泡的变化;四甲基偶氮唑盐还原法检测经自噬特异性抑制剂3-MA预处理后WH-8对细胞生长抑制率的影响;蛋白质印迹法观察WH-8作用下细胞自噬特征性蛋白酵母自噬相关基因1(Beclin 1)和微管相关蛋白1轻链3(LC3)的表达情况。结果WH-8呈时间-剂量依赖性抑制人结肠癌HT-29细胞增殖,48 h平均半数抑制浓度为(12.5±1.3)μmol/L;一定浓度下WH-8可诱导细胞的自噬囊泡增多;3-MA可逆转WH-8对人结肠癌HT-29细胞的生长抑制作用,差异有统计学意义(P<0.05);WH-8可诱导Beclin-1表达上调,且在一定时间内促进LC3Ⅰ向LC3Ⅱ转化。结论WH-8可抑制人结肠癌HT-29细胞增殖并诱导细胞自噬。Objective To initially investigate the effects of curcumin analog WH-8 on the proliferation and autophagy of human colon cancer HT-29 cells.Methods Different concentrations of WH-8 were used to act on HT-29 cells,and MTT method was used to investigate its inhibitory effect on cell growth;fluorescence staining and cell imaging techniques were used to investigate the changes of autophagic vesicles induced by WH-8;MTT method was used to detect the effect of WH-8 on cell growth inhibition rate after pretreatment with autophagy-specific inhibitor 3-MA;western blot was used to investigate the expression of autophagy characteristic proteins[Beclin-1 and microtubule-associated protein 1 light chain 3(LC3)]in WH-8 cells.Results WH-8 inhibited the proliferation of HT-29 cells in a time-dose dependent manner,with an IC50 value of(12.5±1.3)μmol/L in 48 hours;WH-8 at a certain concentration could induce an increase in autophagic vesicles in cells;3-MA could reverse the growth inhibition of WH-8 on human colon cacner HT-29 cells,and the difference is statistically sigificant(P<0.05);WH-8 could induce the up-regulation of the expression of Beclin-1,and promote the transformation from LC3Ⅰto LC3Ⅱwithin a certain period of time.Conclusion The curcumin analog WH-8 could inhibit the proliferation of HT-29 cells and induce autophagy.

关 键 词：姜黄素类似物 结肠癌 人结肠癌HT-29细胞 增殖 自噬 

分 类 号：R735.35[医药卫生—肿瘤] R282.71[医药卫生—临床医学]