鼠李糖脂产量的提高及采油应用研究  

Optimization of fermentation conditions and evaluation of oil displacement potential of an oil producing functional strain

作　　者：冯艳 修建龙[2,3] 伊丽娜[3] 黄立信[2,3] 马原栋[3] 俞理[2,3] FENG Yan;XIU Jian-long;YI Li-na;HUANG Li-xin;MA Yuan-dong;YU Li(University of Chinese Academy of Sciences,Beijing 100049,China;Institute of Percolation Fluid Mechanics,Chinese Academy of Sciences,Langfang 065007,China;PetroChina Research Institute of Petroleum Exploration&Development,Beijing 100083,China)

机构地区：[1]中国科学院大学,北京100049 [2]中国科学院渗流流体力学研究所,河北廊坊065007 [3]中国石油勘探开发研究院,北京100083

出　　处：《应用化工》2023年第3期795-800,共6页Applied Chemical Industry

基　　金：中国石油天然气股份有限公司科学研究与技术开发项目(2021DJ1605)。

摘　　要：对一株铜绿假单胞菌进行发酵条件优化,最优培养基为:菜籽油80 g/L、NaNO_(3)6 g/L、Na_(2)HPO_(4)·12H_(2)O_(3) g/L、KH_(2)PO_(4)1.5 g/L、MgSO_(4)0.36 g/L、FeSO_(4)·7H_(2)O 0.2 g/L、CaCl_(2)0.12 g/L;最适培养条件为:温度37℃,装液量40 mL/250 mL,发酵时间168 h。在最优条件下,鼠李糖脂平均产量可达57.83 g/L,表面张力降到27.89 mN/m。鼠李糖脂在相对苛刻的温度、盐度、pH环境中具有较强的稳定性。在物理模拟驱油实验中,用发酵液进行实验,添加具有增黏性质的生物聚合物黄原胶辅助,建立了一种新的生物驱油体系。5%鼠李糖脂发酵液的生物复合体系采收率可达17.4%,表明其在微生物采油领域有着较大的应用前景。The fermentation conditions of Pseudomonas aeruginosa strain were optimized,the optimal culture medium is obtained as follows:Canola oil 80 g/L,NaNO_(3)6 g/L,Na_(2)HPO_(4)·12H_(2)O_(3) g/L,KH_(2)PO_(4)1.5 g/L,MgSO_(4)0.36 g/L,FeSO_(4)·7H_(2)O 0.2 g/L,CaCl_(2)0.12 g/L.The optimum culture conditions are as follows:temperature 37℃,liquid volume 40 mL/250 mL,fermentation time 168 h.The average rhamnolipid yield is 57.83 g/L and the surface tension is 27.89 mN/m.At the same time,the physicochemical properties of rhamnoid are found to be stable in the environment of relatively harsh temperature,salinity and pH.In the physical simulation experiment,a new bio-oil displacement system is established by using fermentation broth and adding xanthan gum,a bio-polymer with viscous properties.Compared with water flooding,the recovery rate of 5%rhamnoid fermentation broth biocomposite system can be increased by 17.4%,indicating that it has a great application prospect in microbial oil recovery field.

关 键 词：微生物采油 生物表面活性剂 铜绿假单胞菌 鼠李糖脂 生物驱油体系 

分 类 号：TQ09[化学工程] TQ920.6[轻工技术与工程—发酵工程] TE357[石油与天然气工程—油气田开发工程]