E3 ligase Deltex2 accelerates myoblast proliferation and inhibits myoblast differentiation by targeting Pax7 and MyoD,respectively  

作　　者：Qi Zhu Ziyun Liang Shufang Cai Xian Tong Xiaoyu Wang Enru Li Yaosheng Chen Delin Mo 

机构地区：[1]State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou 510260,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第2期250-261,共12页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(Nos.32072697 and 31902134);Laboratory of Lingnan Modern Agriculture Project(No.NZ2021006);the China Agriculture Research System(No.CASR-35).

摘　　要：E3 ubiquitin ligases are closely related to cell division,differentiation,and survival in all eukaryotes and play crucial regulatory roles in multiple biological processes and diseases.While Deltex2,as a member of the DELTEX family ubiquitin ligases,is characterized by a RING domain followed by a C-terminal domain(DTC),its functions and underlying mechanisms in myogenesis have not been fully elucidated.Here,we report that Deltex2,which is highly expressed in muscles,positively regulates myoblast proliferation via mediating the expression of Pax7.Meanwhile,we find that Deltex2 is translocated from the nucleus into the cytoplasm during myogenic differentiation,and further disclose that Deltex2 inhibits myoblast differentiation and interacts with MyoD,resulting in the ubiquitination and degradation of MyoD.Altogether,our findings reveal the physiological function of Deltex2 in orchestrating myogenesis and delineate the novel role of Deltex2 as a negative regulator of MyoD protein stability.

关 键 词：E3 ubiquitin ligases Deltex2 myoblast proliferation and differentiation Pax7 MYOD 

分 类 号：R73[医药卫生—肿瘤]