CRISPR/Cas12a combined with recombinase polymeraseamplification for rapid and sensitive detection of Vibriovulnificus in one tube  

作　　者：Xue Zhang Bo Guo Lihong Yang Chenjie Zhao Yue Wang Yixin Tang Guang Yang Pei Wang Song Gao 

机构地区：[1]Jiangsu Key Laboratory of Marine Biological Resources and Environment,Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening,Jiangsu Ocean University,Lianyungang 222005,China [2]School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第2期322-326,共5页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the Key Natural Science Research Project of the Jiangsu Higher Education Institutions of China(No.20KJA416002);the Jiangsu Funding Program for Excellent Postdoctoral Talent of China(No.2022ZB358);the Research Program of“521 Project”of Lianyungang City of China(No.LYG06521202133);the Open-end Funds of Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening(No.HY202004);the“Blue Project”of Jiangsu Higher Education Institutions of China,and the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.

摘　　要：Vibrio vulnificus is an important foodborne pathogenic bacterium.Contaminated seafood can cause wound infections,septicemia and acute gastroenteritis that can be fatal if not treated appropriately in time.As one of the most dangerous foodborne bacteria,V.vulnificus is responsible for 95%of seafood-related deaths,presenting a major food safety concern[1].With the globalization of food supply chains,regular surveillance of V.vulnificus has become even more important.In this regard,reliable and convenient detection assays are important tools for good control of this pathogen.

关 键 词：CRISPR/Cas VULNIFICUS WOUND 

分 类 号：Q5[生物学—生物化学]