海兰褐蛋鸡盲肠中乳酸菌的分离与鉴定  

Isolation and identification of Lactobacillus from cecum of Hyline brown laying hens

作　　者：孙禹 马艳[1] 王之超 向天翼 任道全[1] SUN Yu;MA Yan;WANG Zhi-chao;XIANG Tian-yi;REN Dao-quan

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300

出　　处：《饲料研究》2023年第5期61-64,共4页Feed Research

基　　金：托木尔峰保护区管理局“托木尔峰陆生脊椎动物本底资源调查项目(项目编号:T20210002)”。

摘　　要：试验旨在从海兰褐蛋鸡盲肠食糜中分离筛选优质乳酸菌。试验采集健康的海兰褐蛋鸡盲肠食糜,将内容物置于无菌生理盐水中,过滤后倍比稀释,采用平板划线分离法进行划线分离,反复提纯,选择疑似菌株,对其进行菌落形态观测、通过革兰氏染色镜检、16S rDNA基因序列分析鉴定,绘制24 h生长、产酸曲线。结果显示,在蛋鸡盲肠食糜中分离出两株菌,分别为唾液乳杆菌(Lactobacillus salivarius)和敏捷乳杆菌(Lactobacillus agilis strain),命名为JZ1和JZ2。两株菌均在10 h达到生长平台期,培养24 h后JZ1的pH值约为4.2,JZ2的pH值约为5.3。研究表明,JZ1具有生长速度快和产酸能力强的优势,耐酸(pH值3.0)、耐高温(45℃)、耐低温(15℃)和耐盐(6.5%NaCl)能力优于JZ2,更适合作为畜牧养殖中的优质菌源。The experiment was to isolate and screen high-quality lactic acid bacteria from caecal chyme of Hyline brown laying hens.In the experiment,the caecal chyme of healthy Hyline brown laying hens was collected,the content were put into sterile normal saline,filtered and diluted in multiples,the plate scribing separation method was used for scribing separation,repeated purification,and the suspected strains were selected for colony morphology observation,microscopic examination after Gram staining,16S rDNA gene sequence analysis and identification,and 24 h growth and acid production curves were drawn.The results showed that two strains of bacteria were isolated from the caecal chyme of laying hens,namely Lactobacillus salivarius and Lactobacillus agilis strain,named JZl and JZ2.Both strains reached the growth plateau in 10 h.After 24 h of culture,the pH value of JZl was about 4.2 and JZ2 was about 5.3.The study indicates that JZ1 has the advantages of fast growth rate and strong acid production capacity.JZ1 is more resistant to acid(pH value 3.0),high temperature(45℃),low temperature(15℃)and salt(6.5%NaCl)than JZ2,which is more suitable to be used as a high-quality bacterial source in animal husbandry.

关 键 词：乳酸菌 分离 鉴定 16S rDNA 

分 类 号：S816.7[农业科学—饲料科学]